June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
Expression of Adenosine Receptors and Their Changes in Age-Related Macular Degeneration
Author Affiliations & Notes
  • Collin Goebel
    Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States
  • Ismail Zaitoun
    Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States
    Macpherson Eye Research Institute, University of Wisconsin-Madison, Madison, Wisconsin, United States
  • Heather Potter
    Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States
  • Nader Sheibani
    Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States
    Macpherson Eye Research Institute, University of Wisconsin-Madison, Madison, Wisconsin, United States
  • Footnotes
    Commercial Relationships   Collin Goebel None; Ismail Zaitoun None; Heather Potter None; Nader Sheibani None
  • Footnotes
    Support  VitreoRetinal Surgery Foundation Grant
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 4618 – F0410. doi:
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    • Get Citation

      Collin Goebel, Ismail Zaitoun, Heather Potter, Nader Sheibani; Expression of Adenosine Receptors and Their Changes in Age-Related Macular Degeneration. Invest. Ophthalmol. Vis. Sci. 2022;63(7):4618 – F0410.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Adenosine receptors (AR) A1, A2A, A2B, and A3 are known to have important roles in the modulation of inflammatory and neurodegenerative processes. However, much remains unknown about their choroidal expression and their function in ocular neurodegenerative diseases including age-related macular degeneration (AMD). This study tested the hypothesis that AR are expressed in the human choroid and changes in their expression occur in patients with AMD.

Methods : Human ocular samples were obtained from the Lion Gift of Sight Eye Bank at the University of Minnesota. Donor eyes with wet AMD, dry AMD, and no AMD were selected. Each set of donor samples was stained with ADORA1, ADORA2A, ADORA2B, and ADORA3 specific antibodies. Each sample was also stained with collagen IV antibody to label the vasculature and DAPI to visualize cellular organization. Samples were then incubated with secondary antibodies and fluorescence microscopy was used to compare the presence and intensity of AR expression.

Results : Receptor A1 demonstrated expression within the retina, particularly the inner plexiform layer (IPL) and outer plexiform layer (OPL). There was also evidence of expression within the choroidal vasculature. Receptor A2A demonstrated expression within the retinal and choroidal vasculature, and its expression was modestly decreased in samples from patients with wet and dry AMD. Receptor A2B demonstrated expression throughout the retina in all samples, particularly the ganglion cell layer, OPL, and IPL. Receptor A2B was also expressed in the retinal and choroidal vasculature. Receptor A3 was expressed primarily within the inner nuclear layer and outer nuclear layer of the retina.

Conclusions : Our results provide further evidence that AR are widely expressed throughout the human retina and choroid, and receptors A1, A2A, and A2B appear to be strongly expressed within the choroidal vasculature. Furthermore, our results suggest decreased receptor A2A expression in patients with both wet and dry AMD may contribute to the pathophysiology of AMD. Additional studies with a larger sample size will be required to further evaluate the contribution of changes in A2A expression to AMD pathology.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

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