Abstract
Purpose :
Otto Haab (1850–1931), Swiss ophthalmologist that contributed with pathological anatomy of the eye, one of than was used to describe tears in the Descemet membrane, thus originated the eponymous Haab striae. The Haab striae are found in congenital glaucoma, trauma as birth injury, contusion, acute hydrops, megalocornea, conical cornea, and with marked AP diameter.
Purpose: to demonstrate the behavior of endothelial mosaic in corneas with Haab striae using contact specular microscopy and compare with normal eye.
Methods :
Ten patients with Haab striae: female 6 and male 4, right eye 6 and left 4, age 6 – 54 and mean 27.7±15 years old. BioOptics contact specular microscope and Bambi 2500 software was used.
Haab striae group (HSG) - eyes with Haab striae (central, near of the HS, further of the HS areas); Control group (CG) - contralateral eyes without striae (central area).
The sampling process of the specular microscopy (images and endotelial cells) was guided by the Cells Analyzer software (USA Patent) to obtain a sampling error smaller than 5% for each especular microsocpy exam.
Descriptive cell data from the sample of the endotelial mosaic acquired by specular microscopy exams: image number, total cell number, and calculated sampling error.
Morphometric data of the endothelial cells: endothelial cell density (ECD), coefficient of variation (CV), and percentage of hexagonal cells (HEX).
Descriptive statistics were used to demonstrate endothelial data and the comparison between the studied groups and areas will be compared by the Student t test at 95% significance level.
Results :
Endothelial samples: CG: 2.8±1.6 images with 328±169 cells per exam and sampling error 3.12±0.86; HSG: 7.2±2.2 images with 393±90 cells per exame and sampling error 2,87±0.50.
Endothelal data of the studied groups (Figure 1A).
Endothelial mosaic areas compared in eyes with Haab Striae (Figure 1B).
The Figure 2 shows the specular microscopy images of the one of the studied Haab stria due to birth injury.
Conclusions :
Haab striae cause continuous and significant loss of endothelial cell density, it's highier at the margins of the Haab striae. The endotelial cell density gradually decreases as it approaches the margins of the Haab striae. Coefficient of variation and percentage of hexagonal cells did not show changes.
This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.