June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
Perfusion Deficits in Diabetes Mellitus without Diabetic Retinopathy Localize to the Perivenular Deep Capillaries in Averaged OCT Angiography
Author Affiliations & Notes
  • Peter L. Nesper
    Department of Ophthalmology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States
  • Ghazi O. Bou Ghanem
    Department of Ophthalmology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States
  • Amani A Fawzi
    Department of Ophthalmology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States
  • Footnotes
    Commercial Relationships   Peter Nesper None; Ghazi Bou Ghanem None; Amani Fawzi NIH grant R01 EY31815 (A.A.F.), Code F (Financial Support), research instrument support by Optovue, Inc., Fremont, California, USA, Code F (Financial Support)
  • Footnotes
    Support  NIH grant R01 EY31815 (A.A.F.), and research instrument support by Optovue, Inc., Fremont, California, USA.
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 2165 – F0228. doi:
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      Peter L. Nesper, Ghazi O. Bou Ghanem, Amani A Fawzi; Perfusion Deficits in Diabetes Mellitus without Diabetic Retinopathy Localize to the Perivenular Deep Capillaries in Averaged OCT Angiography. Invest. Ophthalmol. Vis. Sci. 2022;63(7):2165 – F0228.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We used geometric perfusion deficit (GPD) analysis in OCT angiography (OCTA) to test the hypothesis that early nonperfusion in patients with diabetes mellitus (DM) without diabetic retinopathy (DR) localizes to the deep capillaries near larger vessels.

Methods : After averaging multiple OCTA scans (average 6.7 ± 1.0 scans), we used a semi-automated method to measure GPD, defined as the retinal area located further than 30 μm from the nearest blood vessel, and compared healthy subjects to patients with DM without DR. In the superficial capillary plexus (SCP), deep (DCP) and full retina, we analyzed percent area of GPD in the whole scan as well as in the 100 μm area around arterioles (periarteriolar space), the 100 μm area around venules (perivenular space) and in the area greater than 100 μm from the nearest arteriole or venule (capillary space).

Results : Our initial analysis included 11 eyes of 11 healthy subjects (6 females; age 39.9 ± 11.7 years) and 11 eyes of 11 patients with DM without clinical DR (7 females; age 46.9 ± 16.0 years). The GPD in the DCP was significantly greater in patients with DM without DR compared to healthy subjects (4.0% ± 1.1% versus 3.2% ± 0.8%, respectively; p=0.030), but not in the SCP or full retina (p=0.371 and p=0.094, respectively). Perivenular GPD in the DCP showed the greatest difference (3.0% ± 1.1% in DM versus 2.2% ± 0.7% in healthy; p=0.028), while the periarteriolar and capillary spaces showed no significant difference (p=0.073 and p=0.151, respectively).

Conclusions : We found that perfusion deficits were increased most prominently in the deep capillaries near venules in patients with DM without clinical DR compared to healthy controls. This study suggests that in the macula, early capillary dysfunction in diabetes may preferentially occur in the perivenular compartment of deep capillary plexus.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

 

Figure 1. Geometric perfusion deficits (GPD) by vascular region in OCT angiography. Top Row) Healthy subject. Bottom Row) Patient with diabetes and no retinopathy. A) Averaged superficial capillary plexus. B) Arterioles (red) and venules (blue). C) Averaged deep capillary plexus (DCP). D) GDP (green) of DCP in capillary space with arterioles and venules superimposed. E) GPD of DCP periarteriolar space. F) GPD of DCP perivenular space. Notice larger perivenular GPD in diabetes without retinopathy (F’) (arrowheads).

Figure 1. Geometric perfusion deficits (GPD) by vascular region in OCT angiography. Top Row) Healthy subject. Bottom Row) Patient with diabetes and no retinopathy. A) Averaged superficial capillary plexus. B) Arterioles (red) and venules (blue). C) Averaged deep capillary plexus (DCP). D) GDP (green) of DCP in capillary space with arterioles and venules superimposed. E) GPD of DCP periarteriolar space. F) GPD of DCP perivenular space. Notice larger perivenular GPD in diabetes without retinopathy (F’) (arrowheads).

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