June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
Macula Ganglion Cell Inner Plexiform Layer and Circumpapillary Retinal Nerve Fiber Layer Thicknesses from Widefield Imaging are similar to Standard Scans
Author Affiliations & Notes
  • Varsha Venkata Srinivasan
    University of Houston College of Optometry, Houston, Texas, United States
  • Siddharth Das
    University of Houston College of Optometry, Houston, Texas, United States
  • Nimesh Bhikhu Patel
    University of Houston College of Optometry, Houston, Texas, United States
  • Footnotes
    Commercial Relationships   Varsha Venkata Srinivasan None; Siddharth Das None; Nimesh Patel None
  • Footnotes
    Support  P30 EY007551, R01 EY029229
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 4419 – F0098. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Varsha Venkata Srinivasan, Siddharth Das, Nimesh Bhikhu Patel; Macula Ganglion Cell Inner Plexiform Layer and Circumpapillary Retinal Nerve Fiber Layer Thicknesses from Widefield Imaging are similar to Standard Scans. Invest. Ophthalmol. Vis. Sci. 2022;63(7):4419 – F0098.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Optical coherence tomography (OCT) inner retinal thickness measures of the macula and circumpapillary regions are important for determining onset and progression of experimental glaucoma in the non-human primate (NHP). Standard raster and circumpapillary scans have excellent repeatability but sample a limited region of the posterior pole. The purpose of this experiment was to determine the agreement and repeatability of inner retinal thickness measures from widefield imaging compared to standard scans

Methods : OCT scans (Spectralis HRA+OCT) were acquired from 30 NHPs with healthy eyes. 11 of these animals were imaged on at least 4 separate days to assess repeatability. Standard scans included a 20x20° raster scan centered on the macula, and a 12° diameter circular scan centered on the optic nerve head. Widefield imaging protocol included a 217 line, 55x45° raster scan centered on the macula. Scans were imported into MATLAB, and the inner retinal borders were delineated using a neural network-based image segmentation algorithm. For comparison, retinal nerve fiber layer (RNFL) thickness was determined from an interpolated 12° scan path from widefield thickness maps. For both standard and widefield scans, the ganglion cell inner plexiform layer (GCIPL) thickness was determined for a region of 16 degrees in diameter centered on the fovea.

Results : The average GCIPL thickness from standard raster scans was 72.7±4.3µm, and 73.7±3.7µm from widefield images (bias = -1.0µm, 95% LOA -4.4 to 2.4µm). Average circumpapillary RNFL thickness from the standard circular scan was 113.2±7.3µm, and 114.2±5.8µm for a similar interpolated path from widefield scans (bias = 1.0µm, 95% LOA -6.5 to 8.6µm). Repeatability (2.77xSw) for GCIPL thickness was 2.7µm and 3.2µm, and for RNFL thickness 5.2µm and 4.5µm, for standard and widefield scans respectively.

Conclusions : Inner retinal thickness measures from widefield imaging have good repeatability and are comparable to those measured using standard scans. One of the major advantages of using widefield imaging is that it encompasses most of the visual field sampled in perimetry.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

 

Representative GCIPL and RNFL thickness maps from widefield imaging

Representative GCIPL and RNFL thickness maps from widefield imaging

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×