June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
Differentially expressed tear proteins in Sjögren syndrome keratoconjunctivitis sicca
Author Affiliations & Notes
  • Stephen Paul Yoon
    Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Eduardo Serrano
    Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Zhiyuan Yu
    Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Stephen C Pflugfelder
    Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Cintia S De Paiva
    Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Footnotes
    Commercial Relationships   Stephen Yoon None; Eduardo Serrano None; Zhiyuan Yu None; Stephen Pflugfelder Allergan, Code C (Consultant/Contractor), Dompen, Code C (Consultant/Contractor), Kala, Code C (Consultant/Contractor), Kowa, Code C (Consultant/Contractor), Novartis Pharma AG, Code C (Consultant/Contractor), Senju, Code C (Consultant/Contractor), Santen, Code F (Financial Support), Yuyu, Code F (Financial Support); Cintia De Paiva Yuyu Pharma, Code F (Financial Support), Roche, Code F (Financial Support), Allysta, Code F (Financial Support)
  • Footnotes
    Support  NIH/NEI EY026893, Research to Prevent Blindness, The Hamill Foundation, The Sid Richardson Foundation, and the Mike Hogg Foundation
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 1537 – A0262. doi:
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    • Get Citation

      Stephen Paul Yoon, Eduardo Serrano, Zhiyuan Yu, Stephen C Pflugfelder, Cintia S De Paiva; Differentially expressed tear proteins in Sjögren syndrome keratoconjunctivitis sicca. Invest. Ophthalmol. Vis. Sci. 2022;63(7):1537 – A0262.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To determine if there are significant differences in the concentrations of tear proteins in Sjögren syndrome keratoconjunctivitis sicca (SS KCS) compared to healthy controls.

Methods : Tear samples were collected with unmarked Schirmer strips from 15 SS KCS patients and 21 healthy controls. Tear protein was eluted and the concentration measured. Inflammatory mediators were assayed with a Raybiotech L-507 glass slide array and normalized by strip wetting length. All patients underwent an ocular surface exam to evaluate tear-break-up time (TBUT), corneal fluorescein staining, and conjunctival staining. Symptom assessment questionnaire in dry eye (SANDE) scores were collected for all patients.

Results : 57 of the 507 tear proteins analyzed were significantly upregulated in SS patients compared to controls (Table 1). Spearman correlations showed that all 57 upregulated tear proteins were significantly inversely correlated with TBUT and positively correlated with corneal fluorescein staining, conjunctival staining and SANDE scores.

Conclusions : These findings indicate that hundreds of factors can be assayed in tear proteins collected from a Schirmer strip. The results suggest tear protein concentrations are altered in SS KCS compared to controls. The upregulated tear proteins correlated with clinical measures of dry eye symptoms and disease severity. Tear protein concentrations could serve as important biomarkers for studying pathogenesis and in clinical diagnosis and management of SS KCS.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

 

 

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