June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
Age-related changes in optical density and geometry of human crystalline lens measured with SS-OCT.
Author Affiliations & Notes
  • Ashish Gupta
    Faculty of Physics, Astronomy and Informatics, Uniwersytet Mikolaja Kopernika w Toruniu, Torun, Kujawsko-Pomorskie, Poland
  • Ebrahim Safarian Baloujeh
    Faculty of Physics, Astronomy and Informatics, Uniwersytet Mikolaja Kopernika w Toruniu, Torun, Kujawsko-Pomorskie, Poland
  • Daniel Ruminski
    Faculty of Physics, Astronomy and Informatics, Uniwersytet Mikolaja Kopernika w Toruniu, Torun, Kujawsko-Pomorskie, Poland
  • Alfonso Jimenez-Villar
    Faculty of Physics, Astronomy and Informatics, Uniwersytet Mikolaja Kopernika w Toruniu, Torun, Kujawsko-Pomorskie, Poland
  • Raul Duarte Toledo
    Laboratorio de Óptica, Universidad de Murcia, Murcia, Murcia, Spain
  • Grzegorz Gondek
    Faculty of Physics, Astronomy and Informatics, Uniwersytet Mikolaja Kopernika w Toruniu, Torun, Kujawsko-Pomorskie, Poland
  • Pablo Artal
    Laboratorio de Óptica, Universidad de Murcia, Murcia, Murcia, Spain
  • Ireneusz Grulkowski
    Faculty of Physics, Astronomy and Informatics, Uniwersytet Mikolaja Kopernika w Toruniu, Torun, Kujawsko-Pomorskie, Poland
  • Footnotes
    Commercial Relationships   Ashish Gupta None; Ebrahim Safarian Baloujeh None; Daniel Ruminski None; Alfonso Jimenez-Villar None; Raul Toledo None; Grzegorz Gondek None; Pablo Artal None; Ireneusz Grulkowski None
  • Footnotes
    Support  Foundation for Polish Science (TEAM Programme, #POIR.04.04.00-00-5C9B/17-00). Polish National Science Center (#2017/26/M/NZ5/00849); Agencia Estatal de Investigación (PID2019-105684RB-I00/AEI/10.13039/501100011033); Fundación Séneca (19897/GERM/15); Horizon 2020 Framework Programme (897300, Marie Sklodowska Curie Individual Fellowship)
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 4405 – F0084. doi:
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    • Get Citation

      Ashish Gupta, Ebrahim Safarian Baloujeh, Daniel Ruminski, Alfonso Jimenez-Villar, Raul Duarte Toledo, Grzegorz Gondek, Pablo Artal, Ireneusz Grulkowski; Age-related changes in optical density and geometry of human crystalline lens measured with SS-OCT.. Invest. Ophthalmol. Vis. Sci. 2022;63(7):4405 – F0084.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To demonstrate in vivo three-dimensional imaging of crystalline lens in healthy eyes with SS-OCT. To objectively determine age-related effects on the crystalline lens by studying lens geometry and optical densities of back-scattered signals from sub-layers of the lens.

Methods : This study includes 50 eyes of 50 healthy volunteers (age range: 9-78 years old). Volumetric images of the crystalline lens were acquired for each eye using the SS-OCT system and the optical scatter index was measured using a double-pass system. The radius of curvature (ROC) and thickness of sub-layers of the lens was measured after refraction correction of the segmented layers. The enhanced intensity image is used to measure densitogram with the optical axis as the center of the image and to average the intensity values in the 54 μm region of interest (ROI). The Oxford nomenclature was used as a reference for the segmentation of distinct CL zones and the nucleus (figure 1). The enhanced intensity image is also used to determine the mean scattering index intensity of each layer of the crystalline lens.

Results : The thickness of the whole lens and measured thickness of the cortex increases with age. The mean ROC of the anterior cornea is 7.34 ± 0.24 mm, the posterior cornea is 6.39 ± 0.20 mm, the anterior lens is 10.1 ± 1.7 mm, the anterior nucleus is 4.06 ± 0.37 mm, the posterior nucleus is 3.67 ± 0.33 mm, the posterior lens is 5.90 ± 0.40 mm. ROC of anterior lens interface, anterior nucleus, and posterior nucleus decreases with age. However, there was no statistical correlation of age with ROC measured for the posterior lens (R = 0.15, P = 0.298). Thickness and mean intensity signal of each layer of the crystalline lens and their relationship with the aging of the eyes are presented in figure 2.

Conclusions : The SS-OCT imaging analysis of the lens indicated age-dependent development of sub-layers in the crystalline lens. SS-OCT for lens imaging is a useful tool for performing in vivo fundamental studies on the development and aging of the human lens.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

 

Scheme of densitogram creation. (A) 3-D OCT dataset of a 41 years-old subject. (B) Schematic diagram of the human lens showing different layers together with the ROI in the central area. (C) Resulting densitogram revealing different layers of CL.

Scheme of densitogram creation. (A) 3-D OCT dataset of a 41 years-old subject. (B) Schematic diagram of the human lens showing different layers together with the ROI in the central area. (C) Resulting densitogram revealing different layers of CL.

 

Comparing age with (A) Radii of curvature, (B) thickness, and (C) mean intensity of the crystalline lens and its sub-layers.

Comparing age with (A) Radii of curvature, (B) thickness, and (C) mean intensity of the crystalline lens and its sub-layers.

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