June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
Assessment of retinal blood flow in infants with Retinopathy of Prematurity using Fluorescein Angiography and Laser Speckle Contrast Imaging
Author Affiliations & Notes
  • Tara Balasubramanian
    University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Victoria Chen
    University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Noela Lu
    Ophthalmology, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Kristin Williams
    Ophthalmology, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Moran Roni Levin
    Ophthalmology, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Sripriya Sundararajan
    Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Osamah Saeedi
    Ophthalmology, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Abhishek Rege
    Vasoptic Medical, Inc, Maryland, United States
  • Janet L. Alexander
    Ophthalmology, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Footnotes
    Commercial Relationships   Tara Balasubramanian None; Victoria Chen None; Noela Lu None; Kristin Williams None; Moran Levin None; Sripriya Sundararajan None; Osamah Saeedi Vasoptic Medical, Inc, Code F (Financial Support), Heidelberg Engineering, Inc, Code F (Financial Support), Aerie Pharmaceuticals, Inc, Code F (Financial Support); Abhishek Rege Vasoptic Medical, Inc, Code E (Employment), Vasoptic Medical, Inc, Code I (Personal Financial Interest), Vasoptic Medical, Inc, Code P (Patent); Janet Alexander None
  • Footnotes
    Support  NH Grant R43EY030798
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 2980 – F0221. doi:
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    • Get Citation

      Tara Balasubramanian, Victoria Chen, Noela Lu, Kristin Williams, Moran Roni Levin, Sripriya Sundararajan, Osamah Saeedi, Abhishek Rege, Janet L. Alexander; Assessment of retinal blood flow in infants with Retinopathy of Prematurity using Fluorescein Angiography and Laser Speckle Contrast Imaging. Invest. Ophthalmol. Vis. Sci. 2022;63(7):2980 – F0221.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To determine the preliminary feasibility of assessing retinal blood flow velocities noninvasively in infants with Retinopathy of Prematurity (ROP) using laser speckle contrast imaging (LSCI) via comparison with intravenous fluorescein angiography (IVFA).

Methods : In this ongoing study, 26 subjects (52 eyes) underwent LSCI using XyCAM RI (Vasoptic Medical, Baltimore, MD) and 6 subjects (6 eyes) received IVFA. Three subjects (3 eyes) aged 3 – 6 months that were diagnosed with ROP received both LCSI and IVFA imaging in quick succession. Perfusion status in 13 arteries and veins of interest was analyzed by determining integrated density and time to peak intensity (TPI) from IVFA data using ImageJ and by computing the mean blood flow velocity index (BFVi) from select vessel segments and in the overall field of view at its peak phase in the cardiac cycle from the LSCI data using the XyCAM software. Time to arterial, laminar, and venous filling was also determined. Arterial-venous transit time (AVTT) and TPI from IVFA was compared to LSCI-based BFVi measurements in subjects with paired imaging data.

Results : Mean peak BFVi for select vessels in subjects who received XyCAM imaging (10 eyes) was 7.2 ± 1.6 (arbitrary units). Mean peak BFVi for overall field of view in subjects who received both imaging modalities was 3.9 ± 1.9. Mean peak BFVi of arteries was significantly different from the peak BFVi of veins (p < 0.009). Mean AVTT was 5.4 ± 1.6 seconds and mean TPI was 12.3 ± 0.7 seconds. Among subjects with paired data from IVFA and LSCI, shorter filling times were associated with higher BFVi.

Conclusions : These preliminary findings from a limited cohort of neonates demonstrate the ability to noninvasively assess perfusion using LSCI in a manner that elevated BFVi measurements are associated with faster arterial-venous transit times. Future studies will be needed to establish if noninvasively obtained blood flow information provides insights on ROP severity.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

 

Figure 1. Selection of identical regions of interest on FA and XyCAM images. Vessel type was identified using time-stamped IVFA images (A). Regions were manually selected on XyCAM images for analysis (B) and selected regions were overlayed onto FA images (C) then were registered and aligned using ImageJ.

Figure 1. Selection of identical regions of interest on FA and XyCAM images. Vessel type was identified using time-stamped IVFA images (A). Regions were manually selected on XyCAM images for analysis (B) and selected regions were overlayed onto FA images (C) then were registered and aligned using ImageJ.

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