Abstract
Purpose :
Purpose: To introduce a method for analyzing adaptive optics (AO) imaging data on the full thickness macular hole (FTMH) and compare the cone density at the corresponding retinal regions surrounding the outer edge of para-macular hole edema (OEME) between the eyes with the FTMH versus the contralateral non-pathological eye within individual patients.
Methods :
Method: Twenty-two patients (n=22) between the ages 53 and 73 years were recruited at the Kensington Eye Institute, Toronto. Each patient had one eye diagnosed with FTMH and the contralateral eye with no pathology. The AO images from 44 eyes were captured using rxt1 AO retinal camera. Nine images of each retina was overlapped covering 8°X8° central retina. The raw fragments of 4°X4° images were aligned to create a montage with developer-provided software. ImageJ was used for creating a grid. On the FTMH montage, a circle outlining OEME, and two perpendicular lines from diagonal meridians by 45° crossing the center of the macular hole were drawn. On the montage of the contralateral eye, a circle delineating the foveal avascular zone (FAZ), two lines with the same features as those on FTMH montage crossing the center of FAZ were drawn. The grid was created by overlaying the two lines from each montage. The created grid was overlaid on each montage. And the region of interest (ROIs) were sampled and compared with the guidance of the grid.
Results :
Results: Of raw images from 22 eyes with FTMH, montage creation failed in 15 eyes (15/22, 68%), which was remarkably higher than contralateral eyes (P=0.01). The shifted fragment images were found in 38.8 % of total images taken from the eyes with FTMH, which was significantly higher than contralateral eyes (7.7%) (P=0.001). The cone density in 4 quadrants by OEME was decreased significantly than the contralateral corresponding retina (p=0.0004). Furthermore, the negative correlation between cone density and spacing at same regions was found in FTMH. (r=0.553, CI 95% [-0.79,-0.16], P=0.009).
Conclusions :
Conclusion: The method of grid overlaying on the montage image was applicable to analyzing the AO imaging acquired from eyes with FTMH. Utilizing the grid, we found the cone density in circular retinal regions at the outer edge of ME was significantly decreased in adaptive optics imaging, which indicated that disturbance of the cones may occur outside OEME.
This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.