Abstract
Purpose :
This study is to demonstrate optoretinography of dark adaptation abnormality due to rod dysfunction in the retinal degeneration 10 (rd10) mouse model.
Methods :
Six WT C57BL/6J and six rd10 mice at postnatal 14 days were used for this study. Optical coherence tomography (OCT) was employed for depth resolved morphological characterization of retinal layers. Time-lapse OCT measurement was used for intrinsic signal optoretinography of dark adaptation. Retinal B-scans were acquired in light condition and during 30 minutes of dark adaptation, with 5 min interval.
Results :
Figure 1 shows comparative OCT imaging of the retina under light and dark-adapted conditions, revealing differences primarily within the outer retina part. Further analysis shows that the peak OCT intensity value of inner segment ellipsoid (ISe) band decreased, and the distance between external limiting membrane (ELM) and retinal pigment epithelium (RPE) reduced during the dark adaptation, compared to light condition. It was observed that the relative ISe intensity change was faster and larger in rd10, compared to WT. After 5 min, the relative ISe intensity value has significantly decreased in rd10 compared to the baseline (p < 0.0001); while significant ISe intensity decrease in WT was observed after 15 min (p < 0.05). Furthermore, the ISe intensity peak is split into a1 (left slope) and a2 (right slope). ISe band width has slightly decreased, while the intensity value of a1 both in WT and rd10 significantly dropped (WT P<0.001, Rd10 P<0.01, compared to light condition) and a2 also shown overall decrease.
Conclusions :
In this study, we found that rd10 retina manifest abnormal outer retinal changes during dark adaptation. During dark adaptation, ISe band thickness was reduced in rd10, and peak intensity rapidly decreased compared to WT group.
This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.