June 2022
Volume 63, Issue 7
Open Access
ARVO Annual Meeting Abstract  |   June 2022
In vivo Characterization of Corneal Shear Modulus Under Localized Cross-linking Using Confocal Air-coupled Optical Coherence Elastography
Author Affiliations & Notes
  • Kirill Larin
    University of Houston, Houston, Texas, United States
  • Fernando Zvietcovich
    University of Houston, Houston, Texas, United States
  • Achuth Nair
    University of Houston, Houston, Texas, United States
  • Manmohan Singh
    University of Houston, Houston, Texas, United States
  • Salavat Aglyamov
    University of Houston, Houston, Texas, United States
  • Michael D Twa
    University of Houston, Houston, Texas, United States
  • Footnotes
    Commercial Relationships   Kirill Larin None; Fernando Zvietcovich None; Achuth Nair None; Manmohan Singh None; Salavat Aglyamov None; Michael Twa None
  • Footnotes
    Support  NIH Grant R01EY022362
Investigative Ophthalmology & Visual Science June 2022, Vol.63, 2399 – A0202. doi:
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      Kirill Larin, Fernando Zvietcovich, Achuth Nair, Manmohan Singh, Salavat Aglyamov, Michael D Twa; In vivo Characterization of Corneal Shear Modulus Under Localized Cross-linking Using Confocal Air-coupled Optical Coherence Elastography. Invest. Ophthalmol. Vis. Sci. 2022;63(7):2399 – A0202.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Recently, the riboflavin/UV-A collagen cross-linking (UV-CXL) clinical technique to treat corneal keratoconus has been applied locally in the cornea for patient-specific treatment. We hypothesize that a confocal air-coupled ultrasonic optical coherence elastography (ACUS-OCE) system can detect local changes of corneal elasticity in vivo under localized and global UV-CXL in rabbits.

Methods : ACUS-OCE measurements were made in four Dutch-Belted rabbits (n = 8 corneas) in the following protocol: (1) the left cornea was measured before (untreated: UT) and after half-treated (HT) UV-CXL; and (2) the right cornea was measured before and after full-treated (FT) UV-CXL. The ACUS-OCE setup includes a spectral-domain optical coherence tomography system coaligned with an air-coupled 1 MHz ultrasonic transducer that produced mechanical excitation (Lamb waves) at the apex of the corneas. Corneal thickness and Lamb wave speed were measured along four meridians: superior, inferior, nasal, and temporal. Finally, a modified Rayleigh-Lamb equation was used to convert wave speed into shear modulus.

Results : Lamb wave speed maps (Fig. 1a) along the inferior-superior direction are shown for the UT, HF, and FT corneas with UV-CXL. The inferior meridian (i.e., UV-CXL treated) in the HT cornea case was stiffer (~7.14 m/s) than its superior (untreated) meridian (~5.88 m/s). Moreover, the speed map for the FT case was symmetric and showed a marked speed elevation (~7.66 m/s) compared to the UT case (~5.31 m/s). There was a significant increase in the shear modulus in the inferior meridian of the left HT corneas (p < .001) after localized UV-CXL (Fig. 1b). Similarly, for the right FT corneas, there is a significant increase in shear modulus (p < .001) in all meridians after full UV-CXL.

Conclusions : In this work, we have demonstrated that ACUS-OCE can detect localized untreated and UV-CXL treated regions in the same cornea in vivo. This technology shows great potential for the monitoring and customization of patient-specific UV-CXL treatment.

This abstract was presented at the 2022 ARVO Annual Meeting, held in Denver, CO, May 1-4, 2022, and virtually.

 

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