The effect of SD on the immune-related cell and genes in mouse ELGs.
(A) Diurnal oscillations of the relative abundance of CD4
+ cells by using immunohistochemistry in the ELGs for the non-SD group and SD group at six-hour intervals (non-SD-treated mice:
F = 8.312,
P < 0.001; SD-treated mice:
F = 7.197,
P < 0.001). Each time point shows the median and SEM. *
P < 0.05, ***
P < 0.001.
(B) Average relative abundance of CD4
+ cells of ELGs from non-SD group and SD group. ***
P < 0.001.
(C) Representative immunohistochemistry images (CD4
+ cells) of mouse ELGs at ZT0 from non-SD group and SD group. The sectional view of ELG structure from the non-SD group (
left) and SD group (
right).
Scale bar: 50 µm.
(D) Diurnal oscillations of the relative abundance of CD8
+ cells by using immunohistochemistry in the ELGs for the non-SD group and SD group at six-hour intervals (non-SD-treated mice:
F = 18.638,
P < 0.001; SD-treated mice:
F = 7.446,
P < 0.001). Each time point shows the median and SEM. ***
P < 0.001.
(E) Average relative abundance of CD8
+ cells of ELGs from non-SD group and SD group. ***
P < 0.001.
(F) Representative immunohistochemistry images (CD8
+ cells) of mouse ELGs at ZT0 from non-SD group and SD group. The sectional view of ELG structure from the non-SD group (
left) and SD group (
right).
Scale bar: 50 µm.
(G) Diurnal oscillations of the relative abundance of CD64
+ cells by using immunohistochemistry in the ELGs for the non-SD group and SD group at six-hour intervals (non-SD-treated mice:
F = 9.532,
P < 0.001; SD-treated mice:
F = 4.159,
P < 0.01). Each time point shows the median and SEM. **
P < 0.01, ***
P < 0.001.
(H) Average relative abundance of CD64
+ cells of ELGs from the non-SD group and SD group. ***
P < 0.001.
(I) Representative immunohistochemistry images (CD64
+ cells) of mouse ELGs at ZT0 from the non-SD group and SD group. The sectional view of ELG structure from the non-SD group (
left) and SD group (
right).
Scale bar: 50 µm.
(J) Diurnal oscillations of the relative abundance of CX3CR1
+ cells by using immunohistochemistry in the ELGs for the non-SD group and SD group at six-hour intervals (non-SD-treated mice:
F = 4.239,
P < 0.01; SD-treated mice:
F = 18.920,
P < 0.001). Each time point shows the median and SEM. *
P < 0.05, ***
P < 0.001.
(K) Average relative abundance of CX3CR1
+ cells of ELGs from the non-SD group and SD group. ***
P < 0.001.
(L) Representative immunohistochemistry images (CX3CR1
+ cells) of mouse ELGs at ZT0 from non-SD group and SD group. The sectional view of ELG structure from the non-SD group (
left) and SD group (
right).
Scale bar: 50 µm.
(M) Diurnal oscillations of the relative abundance of γδ
+ T cells by using immunohistochemistry in the ELGs for the non-SD group and SD group at six-hour intervals (non-SD-treated mice:
F = 6.625,
P < 0.001; SD-treated mice:
F = 4.620,
P < 0.01). Each time point shows the median and SEM. **
P < 0.01, ***
P < 0.001.
(N) Average relative abundance of γδ T
+ cells of ELGs from non-SD group and SD group. ***
P < 0.001.
(O) Representative immunohistochemistry images (γδ T
+) of mouse ELGs at ZT0 from the non-SD group and SD group. The sectional view of ELG structure from the non-SD group (
left) and SD group (
right).
Scale bar: 50 µm.
(P) Schematic diagram shows diurnal changes in the number of different immune cells in non-SD-treated mouse ELGs (
left) and SD-treated mouse ELGs (
right). Reproduced from Huang
31.
(Q) Heatmaps of diurnal expression for immune-related genes between the non-SD group and SD group in mouse ELGs. The expression levels of immune-related genes were obtained from RNA-Seq and expression range of DEGs was normalized to ± 3.
(R) The top 10 KEGG pathways enriched histogram of immune-related genes with
Q < 0.05 were displayed.
(S) The PPANs and functional clusters (clusters 1–3) with relevant KEGG pathway of immune-related genes between the non-SD group and SD group in mouse ELGs (
Q < 0.05).