Mouse eyes were dissected and incubated with dispase (15 mg/mL, neutral protease [Dispase], #44439; Cell Signaling Technology, Danvers, MA, USA) overnight at 4°C. Corneal epithelial cell sheets were gently peeled off corneas and placed into 200 to 500 µL of Gibco Trypsin-EDTA (0.25%), phenol red (#25200072; Thermo Fisher Scientific) at 37°C for 10 to 15 minutes to form single-cell suspensions. Trypsin was neutralized by the addition of one volume (200–500 µL, 10 mg/mL) of MilliporeSigma Cabiochem Trypsin Inhibitor, Soybean (#65035100MG; Thermo Fisher Scientific). Cells were pelleted at 500g for 5 minutes and resuspended in defined culture medium (Gibco EpiLife Medium, #MEPI500CA; Thermo Fisher Scientific) supplemented with Gibco Human Corneal Growth Supplement (HCGS, #S0095; Thermo Fisher Scientific), 5 ng/mL Gibco Human EGF Recombinant Protein (EGF, #PHG0313; Thermo Fisher Scientific), Gibco AlbuMAX I Lipid-Rich BSA (#11020; Thermo Fisher Scientific), insulin–transferrin–selenium (ITS-G, 100X, #41400045; Thermo Fisher Scientific), and Gibco Human Transferrin (#0030124SA; Thermo Fisher Scientific). Cells were plated into 65-mm Petri dishes coated with 0.1 mg/mL poly-d-lysine (PDL, #A3890401; Thermo Fisher Scientific) and laminin (EHS sarcoma, mouse, #1243217; Sigma-Aldrich). After 4 days in culture, cells were stripped with Gibco Trypsin–EDTA (0.05%, #25300120; Thermo Fisher Scientific), and replated into 96-well plates coated with PDL/laminin, with 10,000 to 20,000 cells per well in 100 µL of defined culture medium (without growth factors) with or without 25 ng/mL of Fgf10 in PBS (recombinant human FGF-10, #345-FG; R&D Systems, Minneapolis, MN, USA). Cell growth and viability were tested using the Cell Meter Colorimetric WST-8 Cell Quantification Kit (#22771; AAT Bioquest, Pleasanton, CA, USA) for 1, 3, and 7 days in culture according to the manufacturer’s protocol.