Abstract
Presentation Description :
Fluorescent calcium indicators have become ubiquitous tools in neuroscience, allowing functional readout from hundreds of neurons simultaneously. By pairing genetically encoded calcium indicators with fluorescence adaptive optics ophthalmoscopy, we can now visualize retinal ganglion cell activity at the cellular scale in the living eye. We apply this technology in non-human primate to interrogate foveal physiology, confirm vision loss after photoreceptor ablation and demonstrate restored RGC function following optogenetic therapy. Furthermore, by performing these experiments in vivo we can assess RGC function longitudinally, from days to years following injury or therapeutic intervention.
This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.