June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Choroidal Resident Macrophage Depletion can Induce Myopia in Mice
Author Affiliations & Notes
  • Jing Hou
    Department of Ophthalmology, Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
    Laboratory of Photobiology, Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
  • Shin-ichi Ikeda
    Department of Ophthalmology, Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
    Laboratory of Photobiology, Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
  • Kiwako Mori
    Department of Ophthalmology, Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
    Laboratory of Photobiology, Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
  • Heonuk Jeong
    Department of Ophthalmology, Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
    Laboratory of Photobiology, Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
  • Hidemasa Torii
    Department of Ophthalmology, Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
    Laboratory of Photobiology, Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
  • Kazuno Negishi
    Department of Ophthalmology, Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
  • Kazuo Tsubota
    Department of Ophthalmology, Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
    Tsubota Laboratory, Inc., Shinjuku, Japan
  • Toshihide Kurihara
    Department of Ophthalmology, Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
    Laboratory of Photobiology, Keio Gijuku Daigaku Igakubu Daigakuin Igaku Kenkyuka, Shinjuku-ku, Tokyo, Japan
  • Footnotes
    Commercial Relationships   Jing Hou 2022-72596, Code P (Patent); Shin-ichi Ikeda 2022-72596, Code P (Patent); Kiwako Mori None; Heonuk Jeong None; Hidemasa Torii None; Kazuno Negishi None; Kazuo Tsubota Tsubota Laboratory, Inc., Code E (Employment), Tsubota Laboratory, Inc., Code I (Personal Financial Interest), 2022-72596, Code P (Patent); Toshihide Kurihara 2022-72596, Code P (Patent)
  • Footnotes
    Support   None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 851. doi:
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    • Get Citation

      Jing Hou, Shin-ichi Ikeda, Kiwako Mori, Heonuk Jeong, Hidemasa Torii, Kazuno Negishi, Kazuo Tsubota, Toshihide Kurihara; Choroidal Resident Macrophage Depletion can Induce Myopia in Mice. Invest. Ophthalmol. Vis. Sci. 2023;64(8):851.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : It's reported that the ablation of a significant majority of choroidal macrophages has been linked to broad atrophy of the choroidal vasculature and choroid thinning (Yang, X., et al. Elife. 2020). To investigate whether choroidal macrophage depletion leads to myopia due to the choroidal thinning, mouse models were used to evaluate the change of refraction, axial length, and choroidal thickness after clodronate liposome intraperitoneal injection to consume the macrophages in the choroid.

Methods : 3-week-old male C57B6/ J mice were randomly divided into two groups: mice injected with PBS liposome (control group) and mice injected with clodronate liposome (clo-lip group) (n = 12 in each group). Before injection and after 8 days of injections, refraction, axial length, and choroidal thickness were measured by an infrared photorefractor and an SD-OCT system, respectively. Flow cytometry was used to analyze the proportion of choroidal macrophages after 8 days of injections. In previous studies, we observed that choroidal thickness does not increase and tends to be stable in mice from about 8 weeks of age (Zhang, Y., et al. PNAS Nexus. 2022). To rule out that choroidal macrophage depletion can cause myopia independently of the condition of choroidal thickness development, 8-week-old mice were injected with clo-lip or PBS liposome in the same manner, and the changes in refraction, axial length, and choroidal thickness were analyzed 8 days later.

Results : At 3 weeks of age, the clo-lip group showed a higher refractive shift (-7.30±3.61 D vs. +1.17±4.40 D, p< 0.001), axial length elongation (0.08±0.02 mm vs. 0.05±0.02 mm, p< 0.01), and choroidal thinning (-1.56±0.89 mm vs. 1.09±0.51 mm, p< 0.001) compared with the control group. Flow cytometry analysis demonstrated a corresponding reduction of choroidal macrophages after 8 days of clo-lip injection compared with the control group (2.583±0.002% vs. 9.290±0.018%, p< 0.05). In mice 8 weeks of age, in the clo-lip group, refraction shift (-7.52±2.57 D vs. +1.52±4.91 D, p< 0.01), a higher axial length/body weight (0.168±0.015 mm/g vs. 0.155±0.002 mm/g, p< 0.05), and thinner choroidal thickness (-2.39±1.33 mm vs. -0.12±1.48 mm, p< 0.01) were demonstrated compared to the control group.

Conclusions : Clodronate liposome injection causes choroidal macrophage depletion and choroidal thinning, which leads to myopia whether the choroid is developing or not.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

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