Abstract
Purpose :
To assess the antimicrobial activity of Sterileyes fabric against two leading ocular pathogen responsible for eye infection
Methods :
Untreated fabric (control) and antimicrobial fabric (Sterileyes-treated) used for ABmasks were cut into 1cm2 size following aseptic techniques and exposed to Staphylococcus aureus strain 38 with 10-times diluted tryptone soya broth and Pseudomonas aeruginosa 6294 with phosphate buffer saline (PBS). The fabrics were incubated for two hours at 37°C with a gentle shake (120 rpm) three times. After this, fabrics were gently washed with PBS to remove loosely bound bacteria, followed by serial dilutions and plating on nutrient agar plates for viable count determination. Viable bacteria were enumerated as CFU/cm2 fabric material after overnight incubation in 37°C.
Results :
Control fabric showed 5.35±0.07 log and treated fabric material showed 5.05±0.07 log S. aureus adhesion respectively. Similarly, control fabric material showed 5.2±0.14 log and treated fabric material showed 4.75±0.07 log P. aeruginosa adhesion. This indicates that the treated fabric was able to induce 0.3 log (50%) and 0.46 log (65%) reduction to S. aureus and P. aeruginosa adhesion respectively, and these inhibitions were statistically significant (P<0.001).
Conclusions :
Sterileyes-treated fabric can significantly reduce contamination of ocular pathogenic bacteria compared to control fabric. The inhibition induced by the fabric could be utilised to reduce microbial contamination in an ophthalmic clinical setting.
This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.