June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Probiotic Limosilactobacillus reuteri DSM 17938 suppresses corneal barrier dysfunction and conjunctival goblet cell reduction in mice subjected to desiccating stress
Author Affiliations & Notes
  • Laura Schaefer
    Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States
  • Stephen C Pflugfelder
    Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Robert Britton
    Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States
  • Cintia S. De Paiva
    Ophthalmology, Baylor College of Medicine, Houston, Texas, United States
  • Footnotes
    Commercial Relationships   Laura Schaefer Biogaia, Code F (Financial Support), Tenza, Panabio, Mikrovia, Code I (Personal Financial Interest); Stephen Pflugfelder Abbvie, Dompe, Kala, Kowa, Code C (Consultant/Contractor), Dompe, Code F (Financial Support), Immuneyez, Code O (Owner); Robert Britton Probiotech, Norgene, Code C (Consultant/Contractor), Biogaia, Code F (Financial Support), Tenza, Panabio, Mikrovia, Code I (Personal Financial Interest); Cintia De Paiva Spring Discovery, Code C (Consultant/Contractor), Rosche, Aerie Pharmaceuticals, BioAegis Therapeutics, Serpass Biologicals, Code F (Financial Support)
  • Footnotes
    Support  NIH/NEI EY026893 (CSDP), NIH EY002520 (Core Grant for Vision Research Department of Ophthalmology), Research to Prevent Blindness Stein Innovation Award (RAB), Research to Prevent Blindness (Dept. of Ophthalmology), The Hamill Foundation, The Sid Richardson Foundation
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 697. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Laura Schaefer, Stephen C Pflugfelder, Robert Britton, Cintia S. De Paiva; Probiotic Limosilactobacillus reuteri DSM 17938 suppresses corneal barrier dysfunction and conjunctival goblet cell reduction in mice subjected to desiccating stress. Invest. Ophthalmol. Vis. Sci. 2023;64(8):697.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Dry eye is a common multifactorial condition caused by inadequate tear production or tear film instability. Microbes residing in the gut can modulate homeostasis of the ocular surface, and microbial dysbiosis can adversely affect the ocular surface, resulting in inflammation and dry eye. In this study, we investigated the potential of an orally administered probiotic bacterial strain, Limosilactobacillus reuteri DSM 17938, to ameliorate dry eye disease in the desiccating stress (DS) mouse model.

Methods : C57BL/6J mice were treated with an antibiotic cocktail containing ampicillin, gentamicin, vancomycin, neomycin, and metronidazole for 7 days to induce intestinal dysbiosis; stools were sequenced with Illumina 16S sequencing. Mice were then subjected to DS, which entails <25% environmental humidity, wire mesh caging exposed to blowing fans, and 0.5mg/ml scopolamine in drinking water. Mice were gavaged daily during DS with either PBS or 5x10^8 probiotic bacteria. Conjunctival goblet cell density was assessed in formalin-fixed Periodic Acid Schiff (PAS)-stained eye histological sections and quantified using NIS-Elements software. Uptake of Oregon-Green-Dextran (OGD) dye was used to evaluate corneal barrier function.

Results : 16S sequencing confirmed profound intestinal dysbiosis after antibiotic treatment. Goblet cell density after 10 days of DS was preserved with administration of DSM 17938 with 28% more goblet cells per mm as compared to PBS controls (P<0.05). In contrast, goblet cell density was not significantly different with administration of either another L. reuteri strain, ATCC PTA6475, or a butyrate-producing Coprococcus bacterium isolated from a healthy human fecal sample. Follow-up experiments with DSM 17938 administration during 5 days of DS demonstrated improved corneal barrier function; corneal permeability to OGD was 2.4 fold less in mice treated with DSM 17938 compared to control mice treated with PBS (P<0.0001), and was similar to the corneas of non-stressed untreated mice. Goblet cell density was also preserved by DSM 17938 treatment, with goblet cell numbers trending higher in DSM 17938-treated mice.

Conclusions : Oral administration of the probiotic bacterial strain L. reuteri DSM 17938 preserves goblet cell density and corneal barrier function in the desiccating stress mouse model of dry eye disease.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×