June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
1,5-Dicaffeoylquinic acid from Pseudognaphalium affine ameliorates dry eye disease via suppression of inflammation and protection of the ocular surface.
Author Affiliations & Notes
  • Joo Youn Oh
    Seoul National University College of Medicine, Seoul, Korea (the Republic of)
  • Jin Suk Ryu
    Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
  • Chang Ho Yoon
    Seoul National University College of Medicine, Seoul, Korea (the Republic of)
  • Hyun-Jae Jang
    Korea Research Institute of Bioscience and Biotechnology, Cheonju, Korea (the Republic of)
  • Jung Hwa Ko
    Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
  • Kyung-Seop Ahn
    Korea Research Institute of Bioscience and Biotechnology, Cheonju, Korea (the Republic of)
  • Sei-Ryang Oh
    Korea Research Institute of Bioscience and Biotechnology, Cheonju, Korea (the Republic of)
  • Jang-Hee Oh
    Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
  • Jin Ho Jung
    Seoul National University College of Medicine, Seoul, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Joo Youn Oh 10-2022-0027751, Code P (Patent); Jin Suk Ryu None; Chang Ho Yoon 10-2022-0027751, Code P (Patent); Hyun-Jae Jang 10-2022-0027751, Code P (Patent); Jung Hwa Ko None; Kyung-Seop Ahn 10-2022-0027751, Code P (Patent); Sei-Ryang Oh 10-2022-0027751, Code P (Patent); Jang-Hee Oh 10-2022-0027751, Code P (Patent); Jin Ho Jung 10-2022-0027751, Code P (Patent)
  • Footnotes
    Support  This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MSIT) (No. 2019M3A9I3091699 and 2019M3A9I3091690).
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 680. doi:
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      Joo Youn Oh, Jin Suk Ryu, Chang Ho Yoon, Hyun-Jae Jang, Jung Hwa Ko, Kyung-Seop Ahn, Sei-Ryang Oh, Jang-Hee Oh, Jin Ho Jung; 1,5-Dicaffeoylquinic acid from Pseudognaphalium affine ameliorates dry eye disease via suppression of inflammation and protection of the ocular surface.. Invest. Ophthalmol. Vis. Sci. 2023;64(8):680.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : In this study, we investigated the potential of dicaffeoylquinic acids (diCQAs), which are polyphenols obtained from Pseudognaphalium affine (P. affine), as a novel treatment for DED.

Methods : We isolated 1,5-, 3,4-, 3,5- or 4,5-diCQAs from the P. affine methanol extract and confirmed them based on NMR, UV, and MS spectroscopic data. Amongst 1,5-, 3,4-, 3,5- or 4,5-diCQAs, we screened for diCQA isomer(s) effective for ocular surface protection in primary cultures of corneal epithelial cells (CECs). Then we tested the therapeutic efficacy of diCQA isomer(s) in two mouse models for DED: desiccating environmental stress-induced DED and the NOD.B10.H2b mouse model of ocular SjS. Also, we compared the therapeutic effects of diCQA(s) with two commercially-available DED treatments: 0.05% cyclosporine ophthalmic emulsion (Restasis®, Allergan, Irvine, CA) and 0.1% sodium hyaluronate eye drops (Hyalein mini 0.1%®, Santen, Osaka, Japan).

Results : Initial screening showed that, among the diCQAs, 1,5-diCQA significantly inhibited apoptosis and enhanced viability in cultures of human CECs under desiccating hyperosmolar stress. Moreover, 1,5-diCQA protected CECs against hyperosmolar stress by promoting proliferation and downregulating inflammatory activation. Subsequent studies with two murine models of DED revealed that topical 1,5-diCQA administration dose-dependently decreased corneal epithelial defects and increased tear production while repressing inflammatory cytokines and T cell infiltration on the ocular surface and in the lacrimal gland. 1,5-diCQA was more effective in alleviating clinical signs of DED, as compared with two commercially-available dry eye treatments, 0.05% cyclosporine and 0.1% sodium hyaluronate eye drops.

Conclusions : Together, our results demonstrate that 1,5-diCQA isolated from P. affine ameliorates DED through protection of corneal epithelial cells and suppression of inflammation, thus suggesting a novel DED therapeutic strategy based on natural compounds.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

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