June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Characterization of conjunctival microflora and antibiotic sensitivity pattern in patients undergoing cataract surgery
Author Affiliations & Notes
  • Aldo Vagge
    DiNOGMI, Universita degli Studi di Genova, Genova, Liguria, Italy
    IRCCS Ospedale Policlinico San Martino, Genova, Liguria, Italy
  • Diego Ponzin
    Fondazione Banca degli Occhi del Veneto, Mestre, Veneto, Italy
  • Chiara Del Noce
    DiNOGMI, Universita degli Studi di Genova, Genova, Liguria, Italy
    IRCCS Ospedale Policlinico San Martino, Genova, Liguria, Italy
  • Davide Camposampiero
    Fondazione Banca degli Occhi del Veneto, Mestre, Veneto, Italy
  • Marcello Santocono
    Centro Oculistico Dott. Marcello Santocono, Catania, Italy
  • Carlo Traverso
    DiNOGMI, Universita degli Studi di Genova, Genova, Liguria, Italy
    IRCCS Ospedale Policlinico San Martino, Genova, Liguria, Italy
  • Giuseppe Giannaccare
    Ophthalmology, Universita degli Studi Magna Graecia di Catanzaro, Catanzaro, Calabria , Italy
  • Footnotes
    Commercial Relationships   Aldo Vagge None; Diego Ponzin None; Chiara Del Noce None; Davide Camposampiero None; Marcello Santocono None; Carlo Traverso None; Giuseppe Giannaccare None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 593. doi:
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      Aldo Vagge, Diego Ponzin, Chiara Del Noce, Davide Camposampiero, Marcello Santocono, Carlo Traverso, Giuseppe Giannaccare; Characterization of conjunctival microflora and antibiotic sensitivity pattern in patients undergoing cataract surgery. Invest. Ophthalmol. Vis. Sci. 2023;64(8):593.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To characterize the conjunctival flora of consecutive patients scheduled for cataract surgery and to determine the susceptibility profile of isolates to several commonly used topical antibiotics

Methods : Conjunctival swabs were performed in patients scheduled for senile cataract surgery before starting any prophylactic treatment. Swabs were processed for the detection of the microbial growth and for species identification. Selective culture media were used and bacteria were identified by the MicroScan Specialty ID Panels (Beckman Coulter®). Antimicrobial susceptibility for netilmicin, tobramycin, ofloxacin, levofloxacin, moxifloxacin, chloramphenicol, and azithromycin were tested using the Kirby-Bauer disk diffusion method. Susceptibility for oxacillin was useful to identify Methicillin Resistant Staphylococcus aureus (MRSA) and Staphylococcus epidermidis (MRSE).

Results : Data from 88 eyes of 44 patients (19 males, 25 females, mean±SD age: 75±12 years) were included. Among swabs analyzed, 61.4% showed only saprophytic flora, 30.7% showed only potential pathogenic flora and 8.0% showed both. S. epidermidis (20.5%), S. intermedius (18.2%) and S. aureus (14.8%) were the isolates most frequent; MRSA and MRSE accounted for 8.0% and 6.8% of isolates respectively. Less frequently (9%), Gram negative bacteria such as Pseudomonas fluorescent, Serratia marcescens, Moraxella lacunata and Morganella morgani were detected. A high susceptibility was obtained for moxifloxacin, including a 100% sensitivity of Gram negative isolates, and 83-100% of sensitivity for Gram positive. All isolates showed a good profile of sensitivity (67-100%) versus choramphenicol too, with the exception of Pseudomonas spp for its intrinsic resistance to this molecule. Among aminoglycosides, the sensitivity of the isolates was better for netilmicin (50-100%), although resistance levels were reported among Gram positive. The lowest susceptibility profile of Gram positive isolates among the fluoroquinolones tested, was found for ofloxacin (16.7-56.3%).

Conclusions : A significant percentage of eyes candidates for surgery presented potential pathogenic flora alone or in association with saprophytic organisms. Staphylococci were the most frequently isolated bacteria. All isolated organisms showed an excellent sensitivity to both moxifloxacin and chloramphenicol.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

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