June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Characterization of the retinal phenotype in the offspring of maternal obesity
Author Affiliations & Notes
  • Anna Sergeyevna Usoltseva
    Ophthalmology, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States
  • Rui Cheng
    Biochemistry, Wake Forest University School of Medicine, Winston-Salem, North Carolina, United States
  • Jiyang Cai
    Physiology, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States
  • Jian-Xing Ma
    Biochemistry, Wake Forest University School of Medicine, Winston-Salem, North Carolina, United States
  • Shaoning Jiang
    Pediatrics, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States
  • Yan Chen
    Ophthalmology, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States
  • Footnotes
    Commercial Relationships   Anna Usoltseva None; Rui Cheng None; Jiyang Cai None; Jian-Xing Ma None; Shaoning Jiang None; Yan Chen None
  • Footnotes
    Support  PRESBYTERIAN HEALTH FOUNDATION (PHF) team science grant
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 561. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Anna Sergeyevna Usoltseva, Rui Cheng, Jiyang Cai, Jian-Xing Ma, Shaoning Jiang, Yan Chen; Characterization of the retinal phenotype in the offspring of maternal obesity. Invest. Ophthalmol. Vis. Sci. 2023;64(8):561.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Adverse maternal environments caused by obesity have been reported to affect the offspring's long-term health and increase the risk of developing certain metabolic diseases, including type 2 diabetes. How obese pregnancy impacts the visual system remains largely elusive. The current study aims to examine the long-term effects of maternal obesity on the retina of the offspring.

Methods : The obese pregnancy model was established by feeding C57BL/6J mice with a high-fat diet (HFD) for 6~8 weeks before mating, throughout gestation, and lactation. Starting at 8 weeks, the offspring of maternal obese breeders (HFD offspring) or control breeders (Chow offspring) were either fed with the high-fat diet or kept on the standard chow. Visual functions were measured by electroretinogram (ERG), retinal thickness by OCT, and the visualization of the fundus with a Micron IV camera. H&E stained paraffin sections of the posterior eyes were examined for pathological changes. The protein levels of the mitochondrial antioxidant system and energy production in the RPE were assessed by western blot.

Results : We showed that at a young age (2~3 M), the offspring born to the obese breeders had normal visual function as indicated by a-wave, b-wave, and c-wave amplitudes comparable to that of the age-matched Chow offspring group. At middle age (10 ~ 12 M), while no significant body changes were detected, HFD offspring exhibited reductions in all three amplitudes, suggesting dysfunction of the neural retina and the RPE. Consistent with the literature studies, HFD, the prediabetic condition, caused progressive neural damage in the Chow offspring. However, HFD did not further aggravate the retinal phenotype in the HFD offspring. Fundus photography did not reveal noticeable changes in all experimental groups at the young and middle ages. Mitochondrial changes are implicated in diabetes. We identified decreased expression of proteins involved in mitochondrial oxidative stress-defensive network and energy production in the RPE of young HFD offspring.

Conclusions : Our current study showed that obese pregnancy negatively impacted the retina of the offspring and caused changes mimicking prediabetic conditions, which may predispose individuals to develop retinal diseases in late life. RPE mitochondrial changes were an early event in the affected offspring. Targeting mitochondria in the affected offspring may provide a means to diminish the risk.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×