Abstract
Purpose :
Contact Lens (CL) wear is a major risk factor for developing ocular complications such as microbial keratitis (MK), Contact lens-induced peripheral ulcer (CLPU) and Contact lens associated Acute Redeye (CLARE). Gram-negative and gram-positive bacteria are causative organisms for these conditions. Graphene oxide (GO) is a derivative of graphene nanosheet, which is widely regarded as the most promising material for its use in biomedical research. In particular, it has effective antimicrobial activity and shows higher biocompatibility with the conjunction of L-cysteine. This study aimed to synthesize and characterize cysteine graphene oxide (Cys-GO) for antimicrobial activity studies.
Methods :
Synthesized cysteine graphene oxide by using coupling agents like 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) and N-hydroxysuccinimide (NHS) with different concentrations and characterized the synthesized product by Raman Spectroscopy (RS), Fourier-Transform Infrared spectroscopy (FTIR) and UV-Visible Spectroscopy.
Results :
In RS the D band for GO was at 1359cm-1, and the G band at 1591cm-1 were detected, and for Cysteine graphene oxide D band at 1338 cm-1 and the G band at 1604 cm-1 were seen. Enlarging of the area under the D band was noticed, and the band between 2599 and 2623 cm−1 in cysteine–GOs can only be ascribed to the thiol functional group. The amide C = O group was found in the FTIR spectrum of cysteine–GO at 1634 cm−1. The stretching peaks of the C = C bond and that of the C = O of amide have been seen in the 1500–1600 cm−1 region. This can be attributed to the co-emerge of these bonds. A wide N-H stretching band is seen in the 3100–3500 cm−1 region.The Uv-Vis spectra of GO show absorbance at 220 nm, Cysteine at 230nm and CysGO at 210 nm and 280 nm.
Conclusions :
The results confirmed the synthesis of CysGO. Further studies will use the synthesized CysGO to assess the antimicrobial activity and cytotoxicity and their applications.
This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.