June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Anti-proliferative Effects of Protein Arginine Deiminase II inhibition on Retinoblastoma
Author Affiliations & Notes
  • Dong Hyun Jo
    Department of Anatomy and Cell Biology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)
  • Hyo Jung Kim
    Department of Anatomy and Cell Biology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)
  • Sojin Kim
    Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul, Korea (the Republic of)
  • Yong Keun Song
    Department of Medicine, Seoul National University College of Medicine, Seoul, Korea (the Republic of)
  • CHANG SIK CHO
    Fight against Angiogenesis-Related Blindness (FARB) Laboratory, Biomedical Research Institute, Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
  • Hyunkyung Kim
    Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Dong Hyun Jo None; Hyo Jung Kim None; Sojin Kim None; Yong Keun Song None; CHANG SIK CHO None; Hyunkyung Kim None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 1261. doi:
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      Dong Hyun Jo, Hyo Jung Kim, Sojin Kim, Yong Keun Song, CHANG SIK CHO, Hyunkyung Kim; Anti-proliferative Effects of Protein Arginine Deiminase II inhibition on Retinoblastoma. Invest. Ophthalmol. Vis. Sci. 2023;64(8):1261.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : This study investigates the anti-proliferative effects of protein arginine deiminase II (PADI2) on retinoblastoma.

Methods : Two retinoblastoma cell lines, Y79 and WERI-Rb1, were treated with BB-Cl-amidine, a chemical inhibitor of PADI2, or with short hairpin RNA (shRNA) targeting PADI2. The effects of inhibition of PADI2 were evaluated through western blotting, cell cycle assay, and cell apoptosis assay. The orthotopic transplantation mouse model was also treated with either a PADI2 inhibitor or shRNA.

Results : PADI2 inhibition significantly reduced cancer cell growth via the inactivation of p-AKT and its downstream pathway in retinoblastoma cells. Apoptosis was also induced by increasing the expression of cleaved PARP in Y79 and WERI-Rb1 cells. In vivo experiments in the orthotopic transplantation also showed anti-proliferative effects of PADI2 inhibition.

Conclusions : PADI2 inhibition reduced the proliferation of retinoblastoma in vitro and in vivo. We suggest PADI2 inhibition as a novel strategy to treat children with retinoblastoma.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

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