June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Expression of active caspase 3 in the rat lens after in vivo exposure to subthreshold dose of UVR-B.
Author Affiliations & Notes
  • Konstantin Galichanin
    Uppsala Universitet, Uppsala, Sweden
  • Zhaohua Yu
    Uppsala Universitet, Uppsala, Sweden
  • Footnotes
    Commercial Relationships   Konstantin Galichanin None; Zhaohua Yu None
  • Footnotes
    Support  Karin Sandqvists Stiftelse grant
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 1221. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Konstantin Galichanin, Zhaohua Yu; Expression of active caspase 3 in the rat lens after in vivo exposure to subthreshold dose of UVR-B.. Invest. Ophthalmol. Vis. Sci. 2023;64(8):1221.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : The aim of this study is to investigate the time evolution of active caspase 3 in the rat lens after in vivo exposure to subthreshold dose of UVR-B.

Methods : Twenty three six-week-old female albino Sprague-Dawley rats were exposed to subthreshold dose (1 kJ/m2) of UVR-B unilaterally and sacrificed at 24, 41, 70 and 120 hours after exposure. Lenses were enucleated and active caspase 3 protein was detected by Western Blot. The time evolution was then plotted as a function of relative mean difference in active caspase 3 between exposed and nonexposed lenses.

Results : There is expression of active caspase 3 in both exposed and nonexposed lenses but there is no difference in relative mean difference in active caspase 3 between exposed and nonexposed lenses in all four postexposure groups.

Conclusions : Exposure to subthreshold dose of UVR-B does not induce apoptosis in the rat lens in vivo within first 120 hours though there is a non-significant increase of active caspase 3 starting at 70 hours. Increase in sample size might reduce the variation level in expression of active caspase 3 in the rat lenses.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×