June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Local lymphatic intervention to promote graft survival in lamellar keratoplasty
Author Affiliations & Notes
  • Meng Shi
    Vision Science Graduate Program, University of California Berkeley, Berkeley, California, United States
    Herbert Wertheim School of Optometry and Vision Science, University of California Berkeley, Berkeley, California, United States
  • Guangyu Li
    Vision Science Graduate Program, University of California Berkeley, Berkeley, California, United States
    Herbert Wertheim School of Optometry and Vision Science, University of California Berkeley, Berkeley, California, United States
  • Yujia Yang
    Vision Science Graduate Program, University of California Berkeley, Berkeley, California, United States
    Herbert Wertheim School of Optometry and Vision Science, University of California Berkeley, Berkeley, California, United States
  • Lu Chen
    Vision Science Graduate Program, University of California Berkeley, Berkeley, California, United States
    Herbert Wertheim School of Optometry and Vision Science, University of California Berkeley, Berkeley, California, United States
  • Footnotes
    Commercial Relationships   Meng Shi None; Guangyu Li None; Yujia Yang None; Lu Chen None
  • Footnotes
    Support  This work is supported in part by research grants from NIH and University of California at Berkeley (LC).
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 1723. doi:
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    • Get Citation

      Meng Shi, Guangyu Li, Yujia Yang, Lu Chen; Local lymphatic intervention to promote graft survival in lamellar keratoplasty. Invest. Ophthalmol. Vis. Sci. 2023;64(8):1723.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Lamellar keratoplasty (LK) is a procedure that replaces partial thickness cornea without involving the endothelium. We have previously reported that this procedure induces intensive formation of lymphatic vessels and valves. In this study, we investigated whether this event can be regulated by local administration of siRNAs and the effect on transplant survival.

Methods : LK was performed between fully mismatched BALB/c (donor) and Prox-1-GFP (green fluorescent protein) mice in C57BL/6 background (recipient; Kang et al. Sci. Rep. 2016). Dynamic processes of lymphatic vessel and valve formation were observed in vivo using an advanced living imaging system for 6 weeks. Corneal grafts were evaluated by slit lamp microscopy and anterior segment optical coherence tomography (OCT). Results were compared between the siRNA treatment (anti-VEGFR-3 and Itga-9) and control (scrambled siRNA) groups after subconjunctival injection.

Results : Local siRNA treatment against VEGFR-3 and Itga-9 significantly inhibited lymphatic and valve formation associated with LK. The outcomes of lamellar grafts were significantly improved as well.

Conclusions : This study provides a new strategy to manage lymphatic events and transplant rejection in LK.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

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