June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Interferon-γ elicits the ocular surface pathology mimicking dry eye through direct modulation of resident corneal cells
Author Affiliations & Notes
  • Jung Hwa Ko
    Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
  • Seonghwan Kim
    Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
  • Jin Suk Ryu
    Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)
  • Joo Youn Oh
    Seoul National University College of Medicine, Seoul, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Jung Hwa Ko None; Seonghwan Kim None; Jin Suk Ryu None; Joo Youn Oh None
  • Footnotes
    Support  This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (2019M3A9I309169911 and 2021R1A2C3004532).
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 1712. doi:
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    • Get Citation

      Jung Hwa Ko, Seonghwan Kim, Jin Suk Ryu, Joo Youn Oh; Interferon-γ elicits the ocular surface pathology mimicking dry eye through direct modulation of resident corneal cells. Invest. Ophthalmol. Vis. Sci. 2023;64(8):1712.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Here, we investigated the direct effects of IFN-γ on resident corneal cells or on the ocular surface.

Methods : We analyzed the dose-dependent effects of IFN-γ on the survival, inflammatory activation and differentiation of corneal stromal fibroblasts and epithelial cells in vitro, and compared them with those of interleukin (IL)-17, the canonical cytokine of Th17 cells that are implicated in the pathogenesis of various ocular surface disorders alongside IFN-γ-producing T cells. Further, we assessed the time- and dose-dependent effects of subconjunctival IFN-γ administration on the ocular surface including the cornea, conjunctiva and lacrimal glands in mice.

Results : Our results demonstrated that IFN-γ dose-dependently induced cytotoxicity, pro-inflammatory cytokine/chemokine production, and expression of major histocompatibility complex class II and CD40 in cultures of corneal stromal fibroblasts and epithelial cells while increasing myofibroblast differentiation of corneal stromal fibroblasts. In mice, subconjunctival IFN-γ administration caused corneal epithelial defects and stromal opacity in dose- and time-dependent manners while promoting neutrophil infiltration and inflammatory cytokine expression in the cornea. Moreover, IFN-γ reduced aqueous tear secretion and the number of conjunctival goblet cells responsible for mucinous tear production.

Conclusions : Together, our findings suggest that IFN-γ induces the ocular surface changes characteristic of dry eye disease at least in part through its direct effects on resident corneal cells.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

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