June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
In-vivo human corneal Lamb wave dispersion using microliter air-pulse optical coherence elastography
Author Affiliations & Notes
  • Chaitanya Duvvuri
    College of Optometry, University of Houston, Houston, Texas, United States
  • Manmohan Singh
    Department of Biomedical Engineering, University of Houston, Houston, Texas, United States
  • Salavat Aglyamov
    Department of Mechanical Engineering, University of Houston, Houston, Texas, United States
  • Kirill Larin
    Department of Biomedical Engineering, University of Houston, Houston, Texas, United States
  • Michael D Twa
    College of Optometry, University of Houston, Houston, Texas, United States
  • Footnotes
    Commercial Relationships   Chaitanya Duvvuri None; Manmohan Singh None; Salavat Aglyamov None; Kirill Larin Elasteye LLC, Code P (Patent); Michael Twa None
  • Footnotes
    Support  R01 EY02236, P30 EY07551, R01 EY033978, and Humana Endowed Dean's Chair in Optometry
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 1698. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Chaitanya Duvvuri, Manmohan Singh, Salavat Aglyamov, Kirill Larin, Michael D Twa; In-vivo human corneal Lamb wave dispersion using microliter air-pulse optical coherence elastography. Invest. Ophthalmol. Vis. Sci. 2023;64(8):1698.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : The development of reproducible techniques capable of quantifying tissue biomechanical properties with high spatial resolution is important in the early detection of diseases with localized features, such as keratoconus. Characterizing tissue biomechanics with high spatial resolution requires high frequency mechanical stimulation, which induces short wavelength mechanical waves. Unlike previous studies that limited the frequency analysis of air-pulse stimulation to 1 kHz, we used stimulation parameters that allowed us to induce high frequency (up to 5 kHz) mechanical waves in tissue. This improved spatial resolution and quantification of wave dispersion (wave speed as a function of frequency), useful for tissue viscoelastic characterization.

Methods : Mechanical waves induced by a spatiotemporally discrete (900 um, 520 us) air-pulse stimulation were detected using phase sensitive OCT. Frequency analysis of tissue responses induced by air-pulse stimulation was used to compute the mechanical wave dispersion in 350 um-thick tissue-mimicking silicone phantoms and four ex-vivo rabbit corneas treated with 20% dextran (each eye evaluated at IOPs 10, 20, and 30 mmHg). The wave dispersion from air-pulse stimulation was compared to the wave dispersion from air-coupled ultrasound (ACUS) stimulation, which induces mechanical waves at specific frequencies. In-vivo human corneal wave dispersion was measured in nine healthy subjects using air-pulse based OCE.

Results : Bland-Altman analysis revealed good agreement between the wave dispersion obtained from ACUS and that obtained from air-pulse stimulation (mean ± 1.96*SD: 0.006 ± 0.5 m/sec). Corneal wave dispersion in ex-vivo rabbit eyes at 10 mmHg resembled Lamb wave’s A0 mode - speed increased with frequency before plateauing at 3 kHz (range, 3.26 m/s to 3.54 m/s), whereas at higher IOPs of 20 and 30 mmHg wave dispersion resembled Lamb wave’s S0 mode - speed decreased with frequency before plateauing at 3 kHz (range, 4.70 m/s to 6.00 m/s to at 20 mmHg and 6.10 m/s to 8.70 m/s at 30 mmHg). In-vivo human corneal wave dispersion measured with air-pulse based OCE was similar to Lamb wave's S0 mode, with the speed plateauing at 3 kHz (range, 8.50 m/s to 9.90 m/s).

Conclusions : These preliminary findings in thin and dispersive tissues show that air-pulse stimulation can be used to reliably measure Lamb wave dispersion in human corneas in-vivo.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×