June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
HISTOPATHOLOGICAL STUDY OF 150 CASES OF KERATOCONUS
Author Affiliations & Notes
  • Massimo Mazza
    The MUHC-McGill University Ocular Pathology & Translational Research Laboratory, Montreal, Quebec, Canada
  • Sabrina Bergeron
    The MUHC-McGill University Ocular Pathology & Translational Research Laboratory, Montreal, Quebec, Canada
  • Devinder Cheema
    The MUHC-McGill University Ocular Pathology & Translational Research Laboratory, Montreal, Quebec, Canada
  • Jacqueline Coblentz
    The MUHC-McGill University Ocular Pathology & Translational Research Laboratory, Montreal, Quebec, Canada
  • Anne Xuan-Lan Nguyen
    The MUHC-McGill University Ocular Pathology & Translational Research Laboratory, Montreal, Quebec, Canada
  • Ana Beatriz Toledo Dias
    The MUHC-McGill University Ocular Pathology & Translational Research Laboratory, Montreal, Quebec, Canada
  • Angela Fajardo Palomino
    The MUHC-McGill University Ocular Pathology & Translational Research Laboratory, Montreal, Quebec, Canada
  • Miguel N. Burnier
    The MUHC-McGill University Ocular Pathology & Translational Research Laboratory, Montreal, Quebec, Canada
  • Footnotes
    Commercial Relationships   Massimo Mazza None; Sabrina Bergeron None; Devinder Cheema None; Jacqueline Coblentz None; Anne Xuan-Lan Nguyen None; Ana Beatriz Toledo Dias None; Angela Fajardo Palomino None; Miguel Burnier None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 1680. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Massimo Mazza, Sabrina Bergeron, Devinder Cheema, Jacqueline Coblentz, Anne Xuan-Lan Nguyen, Ana Beatriz Toledo Dias, Angela Fajardo Palomino, Miguel N. Burnier; HISTOPATHOLOGICAL STUDY OF 150 CASES OF KERATOCONUS. Invest. Ophthalmol. Vis. Sci. 2023;64(8):1680.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Keratoconus is a bilateral and asymmetrical cornea ectasia. In 2008, a study was conducted on 49 cases of keratoconic corneas to document the prevalence of the most common morphological features. The purpose of this research is to provide an update, with a sample size that is three-fold, to confirm and add to the previous findings reported about the pathophysiology of keratoconus. This will help solidify the pathophysiology of keratoconus and aid physicians in the correct histopathological diagnosis of the disorder early on in development.

Methods : A total of 150 cases of keratoconic corneas were studied, including the previous 49 corneas, from the MUHC – McGill University Ocular Pathology & Translational Research in Montreal, Quebec, Canada. A retrospective study of the corneas, obtained after penetrating keratoplasty, was conducted to document the prevalence of the most common morphological features. Histopathological reports were reviewed to obtain data such as age and sex. Specimens were fixed in 10% buffered paraformaldehyde solution, bisected through the centre of the button, and embedded in paraffin. Sections were stained with haematoxylin and eosin and periodic acid-Schiff for light microscopic examination.

Results : The most common morphological features of the corneas were the following: 87% (N=130) of cases showed signs of epithelial thinning, 91% (N=136) showed breaks in the Bowman’s layer, 65% (N=97) showed compaction of the stromal fibres, 63% (N=94) showed folds in Descemet’s membrane. Less common features were superficial iron deposition with 27% (N=40) of cases, 30% (N=45) showed deep stromal scarring, 29% (N=44) showed epithelial scarring, 31% (N=47) showed epithelial cell loss, and 18% (N=27) showed breaks in Descemet’s membrane. The major differences between the findings are that the prevalence in breaks in Bowman’s layer was found to be higher than epithelial thinning in this study. The previous study documented a prevalence of 71% (N=35) for the breaks in Bowman’s layer and 82% (N=40) for the epithelial thinning.

Conclusions : This much larger sample size confirms the incidence and prevalence of the histopathological signs of the diagnosis of keratoconus first reported in the previous study. These new findings also explain the role of the epithelium and the Bowman’s layer in the early development of this disease and support that changes in the superficial layer of the cornea precede stromal development in keratoconus.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×