June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Functional Analysis of Glia Cells in RGC using coculture system
Author Affiliations & Notes
  • Ji-Ae Ko
    Ophthalmology, Hiroshima Daigaku Daigakuin Ikei Kagaku Kenkyuka, Hiroshima, Hiroshima, Japan
  • Akira Minamoto
    Ophthalmology, Hiroshima Daigaku Daigakuin Ikei Kagaku Kenkyuka, Hiroshima, Hiroshima, Japan
  • Hideaki Okumichi
    Ophthalmology, Hiroshima Daigaku Daigakuin Ikei Kagaku Kenkyuka, Hiroshima, Hiroshima, Japan
  • Kazuyuki Hirooka
    Ophthalmology, Hiroshima Daigaku Daigakuin Ikei Kagaku Kenkyuka, Hiroshima, Hiroshima, Japan
  • Yoshiaki Kiuchi
    Ophthalmology, Hiroshima Daigaku Daigakuin Ikei Kagaku Kenkyuka, Hiroshima, Hiroshima, Japan
  • Footnotes
    Commercial Relationships   Ji-Ae Ko None; Akira Minamoto None; Hideaki Okumichi None; Kazuyuki Hirooka None; Yoshiaki Kiuchi None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 1573. doi:
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    • Get Citation

      Ji-Ae Ko, Akira Minamoto, Hideaki Okumichi, Kazuyuki Hirooka, Yoshiaki Kiuchi; Functional Analysis of Glia Cells in RGC using coculture system. Invest. Ophthalmol. Vis. Sci. 2023;64(8):1573.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We have reported that mesencephalic astrocyte derived neutrotrophic factor (MANF) secreted from midbrain is involved in neuroprotection by co-culture system. We have now examined the effects of glia cells on neuroprotection using applied co-culture system.

Methods : Primary retinal ganglion cells from 4-5days rats after birth and glia cells from rats were cocultured using 3D-transwell or vitrigel culture system. Using RT-PCR and immunoblot analysis, multi array, the expression levels of the several survival markers of retinal ganglion cells were studied. Also, the extension of neurites of retinal ganglion cells after coculture was examined by immunoflurorescence analysis.

Results : Co-culture of glia cells and RGCs, no remarkable neuroprotective function by in normal conditioned RGCs (21% O2 ). But, in additional oxidative stress (hypoxia, 1% O2), we found increase in the expression of neural markers and extension of neurites after coculture with glia cells compared to without that in retinal ganglion cells. So, the coculture with glia cells was blocked the damage from oxidative stress in retinal ganglion cells.

Conclusions : Co-Culture with retinal glia cells suggested the possibility of various signaling pathways involved in in vivo neuroprotection. In the future, based on this signal pathway, we will clarify the factors that involved in the survival of RGCs

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

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