June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Suppression of the growth of lase-induced CNV by lacking S1Pr3 is not exaggerated by pharmacological inhibition of S1Pr2 in mice.
Author Affiliations & Notes
  • Hiroki Iwanishi
    Ophthalmology, Wakayama Kenritsu Ika Daigaku, Wakayama, Wakayama, Japan
  • Takayoshi Sumioka
    Ophthalmology, Wakayama Kenritsu Ika Daigaku, Wakayama, Wakayama, Japan
  • Shingo Yasuda
    Ophthalmology, Wakayama Kenritsu Ika Daigaku, Wakayama, Wakayama, Japan
  • Kousuke NIshi
    Ophthalmology, Wakayama Kenritsu Ika Daigaku, Wakayama, Wakayama, Japan
  • Kana Ichikawa
    Ophthalmology, Wakayama Kenritsu Ika Daigaku, Wakayama, Wakayama, Japan
  • Masayasu Miyajima
    Ophthalmology, Wakayama Kenritsu Ika Daigaku, Wakayama, Wakayama, Japan
  • Yuka Okada
    Ophthalmology, Wakayama Kenritsu Ika Daigaku, Wakayama, Wakayama, Japan
  • Shizuya Saika
    Ophthalmology, Wakayama Kenritsu Ika Daigaku, Wakayama, Wakayama, Japan
  • Footnotes
    Commercial Relationships   Hiroki Iwanishi None; Takayoshi Sumioka None; Shingo Yasuda None; Kousuke NIshi None; Kana Ichikawa None; Masayasu Miyajima None; Yuka Okada None; Shizuya Saika None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 2094. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Hiroki Iwanishi, Takayoshi Sumioka, Shingo Yasuda, Kousuke NIshi, Kana Ichikawa, Masayasu Miyajima, Yuka Okada, Shizuya Saika; Suppression of the growth of lase-induced CNV by lacking S1Pr3 is not exaggerated by pharmacological inhibition of S1Pr2 in mice.. Invest. Ophthalmol. Vis. Sci. 2023;64(8):2094.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To evaluate how systemic sphigosine-1-phosphate receptor 2 (S1Pr2) antagonist treatment affects on the impairment of laser-induced choroidal neovascularization (CNV) in S1Pr3 deficient mice. We previously reported that gene deletion of S1Pr3 suppressed the development of laser-induced CNV in association with upregulation of S1Pr2 in tissue (2019 ARVO, 2023 ISER).

Methods : C57BL/6-background S1Pr3-null (KO) mice of 6-8 week-old were used. Argon laser-photocoagulation (4 shots, 80 μm spot size, 0.1 sec. duration, 200 mW) was performed in both eyes of KO (n = 10) mice. An S1Pr2 antagonist, JTE-013(1.2 mg/kg, n=5) or vehicle (0.1% DMSO in PBS 0.1ml, n=5) were administered daily by i.p. injection until euthanasia at day 14. The size of CNV was measured at 2 weeks by software-assisted fluorescence angiography after sacrifice and enucleation.

Results : As previously reported S1Pr3 deficiency reduced the size of CNV. The CNV size in S1Pr3 deficient mice was not further altered by the systemic administration of S1Pr2 antagonist (JTE-013) as compared with the vehicle group.

Conclusions : Suppression of the growth of lase-induced CNV was not further affected by inhibition of S1Pr2, suggesting blocking S1Pr3 might be a promising strategy in the medical treatment of neovascular macropathy.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×