Abstract
Purpose :
To compare two different concentrations of fluorescein, 10% and 25%, after oral administration in order to perform Retinal Fluorescein Angiography (FA). With this study we seek to obtain: 1) Initial fluorescence time. 2) Peak phase of maximal fluorescence (MaxF). 3) Comparison of overall fluorescence. 4) Comparison of fluorescence by time.
Methods :
Cross-sectional, comparative study. The study was approved by the Institutional Review Board and was adherent to the Declaration of Helsinki. Nineteen overall systemically and ophthalmologically healthy volunteers were invited to participate. Volunteers were randomized into two groups: 10% and 25% of 4 ml of orally administered fluorescein. All participants had at least 7 hours of fasting. The concentration was blinded from volunteers and from the examiner. One eye of each participant was selected for retinal FA under pupillary dilation.
After oral administration images of the selected eye was imaged every minute from 0 to 10 minutes, then every five minutes, from 10 to 30 minutes, then every ten minutes from 30 to 60 minutes. Participants were asked to report any adverse events they experienced.
Images were obtained using the Clarus 700 FA acquisition. All images were exported and processed using ImageJ software. A histogram of the distribution of grey values was used to obtain the mean grey value as a measurement of fluorescence.
Results :
Nineteen eyes of 19 volunteers were analyzed, 10 in group 1 (10%), 9 in group 2 (25%). Mean overall fluorescence for group 1: 32.55 pixels (p) (95% confidence interval [CI] 28.24-36.86; standard deviation [SD] 24.75). Overall fluorescence for group 2: 38.62p (95% CI 33.14-44.11; SD 29.68). Comparison of fluorescence by time: Mann-Whitney U 2957.00, Z-Score -2.020, P= 0.043.
Initial fluorescence for group 1: 11.5 seconds (s) (95% CI 2.98-20.01, SD 11.90). Group 2: 8.22s (95% IC 3.81-12.63, DE 5.7). Comparison of initial fluorescence: Mann-Whitney U: 40.00, Z-score: -0.411, P: 0.681.
MaxF for group 1: 62.79p (95% IC 53.70-71.89, SD 12.70). Group 2: 72.68p (95% CI 58.11-87.25, SD 18.95). Comparison of MaxF: Mann-Whitney U: 27.00. Z-Score: -1.42, P=.142.
No adverse events were reported.
Conclusions :
While oral FA can be obtained with two concentrations of fluorescein, 25% demonstrated more fluorescence throughout the study. There were no differences in initial and peak phases.
This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.