June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Myopia-induced changes in the choroid mirror the microscopic biomechanical changes in the adjacent sclera
Author Affiliations & Notes
  • Kazuyo Ito
    Institute of Engineering, Tokyo Noko Daigaku, Tokyo, Japan
  • Jonathan Mamou
    Department of Radiology, Weill Cornell Medicine, New York, New York, United States
  • Cameron Hoerig
    Department of Radiology, Weill Cornell Medicine, New York, New York, United States
  • Yee Shan Dan
    Singapore Eye Centre, Duke-NUS Medical School, Singapore National Eye Centre, Singapore, Singapore, Singapore
  • Sally A McFadden
    Vision Sciences, The University of Newcastle Hunter Medical Research Institute, New Lambton, New South Wales, Australia
  • Quan V Hoang
    Singapore Eye Centre, Duke-NUS Medical School, Singapore National Eye Centre, Singapore, Singapore, Singapore
    Department of Ophthalmology, Columbia University Irving Medical Center, New York, New York, United States
  • Footnotes
    Commercial Relationships   Kazuyo Ito None; Jonathan Mamou None; Cameron Hoerig None; Yee Shan Dan None; Sally McFadden None; Quan Hoang None
  • Footnotes
    Support  This work was supported in part by the National Medical Research Council, Singapore (CSIRG/MOH-000531/2021, QVH), the National Institute of Health, USA (EB028084 (JM), EY023595 (QVH), G1401348 (SAM)), Hunter Medical Research Institute (G1400967 (SAM)), and JSPS Grant-in-aid 22K20502 (KI).
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 2857. doi:
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    • Get Citation

      Kazuyo Ito, Jonathan Mamou, Cameron Hoerig, Yee Shan Dan, Sally A McFadden, Quan V Hoang; Myopia-induced changes in the choroid mirror the microscopic biomechanical changes in the adjacent sclera. Invest. Ophthalmol. Vis. Sci. 2023;64(8):2857.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Retina-derived growth signals relayed from the choroid to the sclera cause remodeling of the extracellular matrix, resulting in myopic ocular elongation. However, no studies have assessed changes in choroidal mechanical properties during myopia progression. The present study utilized 7-µm-resolution scanning acoustic microscopy (SAM) to assess the mechanical properties of choroids in guinea pig eyes with form-deprivation (FD) induced myopia.

Methods : Eleven 1-week-old guinea pigs underwent unilateral FD for 1 week (resulting in -3 to -9.3D myopia). Eyes were enucleated, flash-frozen and 12-μm-thick serial cryosections were obtained in either a vertical (superior/inferior) or horizontal (nasal/temporal) orientation across the posterior pole. Specimens were scanned with SAM and two-dimensional maps of bulk modulus (K), mass density (rho) and choroidal thickness were calculated from values averaged across each sample region (approximately every 200 µm). Paired t-tests were performed to determine statistical differences between the: FDM versus fellow-control eyes, choroid versus sclera tissue, and proximal versus distal regions. Univariate regression analysis was performed to assess the association between choroidal biomechanical parameters (K or rho) and 1) choroidal thickness, 2) the level of induced myopia, and 3) corresponding scleral biomechanical parameters.

Results : In untreated eyes, K was significantly larger and rho was significantly lower in proximal compared to distal regions (p=0.016, p<0.001 respectively). In contrast, in myopic eyes, differences in rho between proximal and distal sections did not reach statistical significance (p = 0.379). Choroidal and scleral K were not correlated in control eyes, except in the SUP-proximal region (R=0.36, p=0.016). In contrast, after 1 week of FDM, choroidal and scleral K became locally correlated (R=0.45, p<0.001) showing that local stiffness was similar between adjacent choroidal and scleral samples. Choroidal and scleral rho showed significant positive correlations in both control and FDM eyes.

Conclusions : Biomechanical changes observed in the choroid of myopic eyes were mirrored in the adjacent sclera. These new findings suggest that choroidal remodeling may accompany myopia and reveal the source of the signals that cause scleral remodeling in myopia.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

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