June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Exogenous Metabolites Associated with Age-related Macular Degeneration (AMD)
Author Affiliations & Notes
  • Ines Lains
    Retina, Massachusetts Eye and Ear, Boston, Massachusetts, United States
  • Kevin Mendez
    Brigham and Women's Hospital, Boston, Massachusetts, United States
  • Joao Gil
    Associacao para a Investigacao Biomedica e Inovacao em Luz e Imagem, Coimbra, Coimbra, Portugal
    Faculdade de Medicina da Universidade de Coimbra, Portugal
  • Joana Providencia
    Associacao para a Investigacao Biomedica e Inovacao em Luz e Imagem, Coimbra, Coimbra, Portugal
    Faculdade de Medicina da Universidade de Coimbra, Portugal
  • Archana Nigalye
    Retina, Massachusetts Eye and Ear, Boston, Massachusetts, United States
  • Raviv Katz
    Retina, Massachusetts Eye and Ear, Boston, Massachusetts, United States
  • Patrícia Barreto
    Associacao para a Investigacao Biomedica e Inovacao em Luz e Imagem, Coimbra, Coimbra, Portugal
  • Rachel S Kelly
    Brigham and Women's Hospital, Boston, Massachusetts, United States
  • Ivana K Kim
    Retina, Massachusetts Eye and Ear, Boston, Massachusetts, United States
  • John B Miller
    Retina, Massachusetts Eye and Ear, Boston, Massachusetts, United States
  • Demetrios G. Vavvas
    Retina, Massachusetts Eye and Ear, Boston, Massachusetts, United States
  • Jessica Lasky-Su
    Brigham and Women's Hospital, Boston, Massachusetts, United States
  • Rufino Silva
    Associacao para a Investigacao Biomedica e Inovacao em Luz e Imagem, Coimbra, Coimbra, Portugal
    Faculdade de Medicina da Universidade de Coimbra, Portugal
  • Joan W Miller
    Retina, Massachusetts Eye and Ear, Boston, Massachusetts, United States
  • Deeba Husain
    Retina, Massachusetts Eye and Ear, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Ines Lains None; Kevin Mendez None; Joao Gil None; Joana Providencia None; Archana Nigalye None; Raviv Katz None; Patrícia Barreto None; Rachel S Kelly None; Ivana Kim Biophytis, Code C (Consultant/Contractor), Kodiak Sciences, Code C (Consultant/Contractor), Allergan, Code R (Recipient); John Miller Alcon, Code C (Consultant/Contractor), Topcon, Code C (Consultant/Contractor), Allergan, Code C (Consultant/Contractor), Carl Zeiss, Code C (Consultant/Contractor), Sunovion, Code C (Consultant/Contractor), Genentech, Code C (Consultant/Contractor); Demetrios Vavvas Olix Pharma, Valitor, Twenty Twenty, Sumitomo/Sunovion, Cambridge Polymer Group, Code C (Consultant/Contractor), Drusolv Therapeutics, Code P (Patent); Jessica Lasky-Su None; Rufino Silva ABBVIE, Alimera, Bayer, Novartis, THEA, Roche, Novo Nordisk, Code C (Consultant/Contractor); Joan Miller Sunovion; KalVista Pharmaceuticals; ONL Therapeutics, Code C (Consultant/Contractor), Lowy Medical Research Institute, Code F (Financial Support), Ciendias Bio, Code I (Personal Financial Interest), Drusolv Therapeutics; Valeant Pharmaceuticals/ Mass. Eye and Ear; ONL Therapeutics, Code P (Patent), Aptinyx, Inc; Sunovion; Kalvista Pharmaceuticals; ONL Therapeutics; Valeant Pharmaceuticals/ Mass. Eye and Ear, Code R (Recipient), Aptinyx, Inc, Code S (non-remunerative); Deeba Husain Genentech, Allergan, Novartis and Omeicos therapeutics, Code C (Consultant/Contractor)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 2759. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Ines Lains, Kevin Mendez, Joao Gil, Joana Providencia, Archana Nigalye, Raviv Katz, Patrícia Barreto, Rachel S Kelly, Ivana K Kim, John B Miller, Demetrios G. Vavvas, Jessica Lasky-Su, Rufino Silva, Joan W Miller, Deeba Husain; Exogenous Metabolites Associated with Age-related Macular Degeneration (AMD). Invest. Ophthalmol. Vis. Sci. 2023;64(8):2759.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Metabolomics can provide insights into the pathophysiology of multifactorial diseases. We and others have shown that patients with age related macular degeneration (AMD) have distinct metabolomic profiles. However, studies performed to date have primarily focused on endogenous metabolites. Given the known multifactorial nature of AMD, this work aimed to examine associations between exogenous plasma and urinary metabolomic profiles and AMD.

Methods : Cross-sectional study including patients with AMD and a control group from Boston, US and Coimbra, Portugal (PT). All participants were imaged with color fundus photographs that were used for AMD grading. The eye with the most advanced AMD stage was used for analysis. Fasting plasma and urine samples were collected and used for metabolomic profiling (Ultrahigh Performance Liquid Chromatography – Mass Spectrometry, Metabolon, Inc). Multivariate logistic (outcome AMD/no AMD) and ordinal logistic (outcome stages of disease) regression models were performed accounting confounding. Each cohort was analyzed separately and then results were combined via meta-analysis.

Results : We included 197 subjects from Boston, US (n=150 AMD) and 297 from Coimbra, PT (n= 244 AMD), and data on 115 plasma and 225 urinary exogenous metabolites. In the Boston cohort the most consistent associations were seen with vanilloylglycine, with both plasma and urine levels showing an association with AMD stages, and urine levels also with AMD diagnosis (p<0.006). In Coimbra, 6 plasma and 8 urine exogenous metabolites showed significant associations with AMD diagnosis and AMD stages accounting for multiple comparisons. In this cohort, pyrraline, an advanced glycation end product, showed the most consistent results, with both urine and plasma levels showing associations with AMD diagnosis and AMD stages (p< 0.002). Both vanilloylglycine and pyrraline have known correlations with oxidative stress. Pyrraline has also been linked to neurodegenerative diseases. After multiple comparisons, no significant associations were seen in the meta-analysis.

Conclusions : To our knowledge, we present the first analysis that focused on exogenous metabolite associations with AMD. This is important as it provides information on possible modifiable risk strategies. The lack of unifying results in our both cohorts is likely related to the distinct habits of US and PT populations.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×