June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Efficacy of spearmint (Mentha spicata L.) extract as nutritional support in a rat model of hypertensive glaucoma
Author Affiliations & Notes
  • Rosario Amato
    Dipartimento di Biologia, Universita degli Studi di Pisa, Pisa, Toscana, Italy
  • Alessio Canovai
    Dipartimento di Biologia, Universita degli Studi di Pisa, Pisa, Toscana, Italy
  • Samanta Maci
    Kemin Human Nutrition and Health, Kemin Foods L.C., Lisbon, Portugal
  • Maurizio Cammalleri
    Dipartimento di Biologia, Universita degli Studi di Pisa, Pisa, Toscana, Italy
  • Massimo Dal Monte
    Dipartimento di Biologia, Universita degli Studi di Pisa, Pisa, Toscana, Italy
  • Footnotes
    Commercial Relationships   Rosario Amato None; Alessio Canovai None; Samanta Maci None; Maurizio Cammalleri None; Massimo Dal Monte Kemin Foods L.C., Code F (Financial Support)
  • Footnotes
    Support  Kemin Foods L.C., Des Moines, IA, USA
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 2586. doi:
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      Rosario Amato, Alessio Canovai, Samanta Maci, Maurizio Cammalleri, Massimo Dal Monte; Efficacy of spearmint (Mentha spicata L.) extract as nutritional support in a rat model of hypertensive glaucoma. Invest. Ophthalmol. Vis. Sci. 2023;64(8):2586.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Glaucoma is characterized by the gradual loss of retinal ganglion cells (RGCs) and optic nerve damage leading to vision loss. Besides the well-established role of intraocular pressure (IOP) elevation in RGC degeneration, recently reduction of oxidative stress and inflammation have emerged as promising targets for optimizing the management of glaucoma. The purpose of the present study is to assess the efficacy of a proprietary spearmint extract (Neumentix® (SPE), Kemin Foods L.C, USA) with reported brain antioxidant and anti-inflammatory benefits, in preserving RGC activity and viability in a rat model of hypertensive glaucoma.

Methods : Ocular hypertension was induced in Sprague-Dawley rats (N=30) through intracameral injection of 15µl of 2% methylcellulose (MCE). Vehicle or SPE at 46.5 mg/Kg or 93 mg/Kg dose was administered daily by oral gavage 14 days before and after MCE injection. An additional group of animals not receiving MCE nor study product (N=10) represent the control group. IOP was daily monitored during the study period. At the endpoint, rats underwent electroretinogram (ERG) recordings, then explanted retinas were analyzed for RGC density and for multiple markers of neural trophism, oxidative stress and inflammation.

Results : Oral supplementation with SPE did not influence the MCE-induced IOP elevation but exerted a dose-dependent preservation of RGC-related ERG responses. SPE effect on RGC function correlated with an improved cell viability and maintenance of RGC density as well as preservation of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). SPE also counteracted the IOP-induced oxidative stress, with a dose-dependent decrease in multiple oxidative markers, promotion of endogenous antioxidant response and inhibition of inflammatory processes. All the above mentioned SPE results were statistically significant vs vehicle and between doses (p<0.01 for all). Notably, for some of the parameters, assessed high-dose SPE was also not statistical different from the non-MCE control.

Conclusions : These findings emphasize the IOP-independent antioxidant, anti-inflammatory and neuroprotective effects of SPE, supporting its possible use, alone or in combination with other ingredients, for the complementary nutritional approach in support to hypotensive treatments for the clinical management of glaucoma.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

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