June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Effect of short-term nicotinamide supplementation on inner retinal function in dogs with ADAMTS10-open-angle glaucoma (ADAMTS10-OAG)
Author Affiliations & Notes
  • Emily Lael Halsmer
    Lincoln Memorial University College of Veterinary Medicine, Harrogate, Tennessee, United States
    Michigan State University College of Veterinary Medicine, East Lansing, Michigan, United States
  • Cheyenne DeQuattro
    Michigan State University College of Veterinary Medicine, East Lansing, Michigan, United States
  • Sichao Wang
    Michigan State University Center for Statistical Training and Consulting, East Lansing, Michigan, United States
  • Christine Harman
    Michigan State University College of Veterinary Medicine, East Lansing, Michigan, United States
  • Amanda Anderson
    Michigan State University College of Veterinary Medicine, East Lansing, Michigan, United States
  • Pete Williams
    Clinical Neuroscience, Karolinska Institutet, Stockholm, Stockholm, Sweden
  • Andras M Komaromy
    Michigan State University College of Veterinary Medicine, East Lansing, Michigan, United States
  • Footnotes
    Commercial Relationships   Emily Halsmer None; Cheyenne DeQuattro None; Sichao Wang None; Christine Harman None; Amanda Anderson None; Pete Williams None; Andras Komaromy Reichert Technologies, Code C (Consultant/Contractor), PolyActiva Pty. Ltd., Code F (Financial Support), CRISPR Therapeutics, Code F (Financial Support), Advanced Ophthalmics LLC, Code F (Financial Support)
  • Footnotes
    Support  NIH grants R01-EY025752 & R01-EY032478, BrightFocus Foundation grant G2022007S, Boehringer Ingelheim Veterinary Scholars grant to Michigan State University, Vetenskapsrådet (2018-02124)
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 2585. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Emily Lael Halsmer, Cheyenne DeQuattro, Sichao Wang, Christine Harman, Amanda Anderson, Pete Williams, Andras M Komaromy; Effect of short-term nicotinamide supplementation on inner retinal function in dogs with ADAMTS10-open-angle glaucoma (ADAMTS10-OAG). Invest. Ophthalmol. Vis. Sci. 2023;64(8):2585.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Previous studies suggest nicotinamide (NAM) supplementation may have a beneficial effect on inner retinal function in glaucomatous patients. This masked, randomized study aimed to demonstrate the effect of 7-week NAM supplementation on inner retinal function using electroretinography (ERG) in ADAMTS10-OAG dogs.

Methods : Ten ADAMTS10-OAG dogs (6 females & 4 males; median age: 4.3 yrs, range: 4.1-6.6 yrs) were enrolled. Five dogs (Group 1) were supplemented orally with 1,500 mg NAM daily. The other 5 dogs served as controls (Group 2) and received sham treatment. Photopic full-field ERGs were recorded at baseline, treatment weeks 5 & 7, and at 13 weeks (washout) using red stimulation of varying intensities (0.07, 0.90, 1.74, 2.57, 3.40, 4.00 cd.s/m2) on blue background (25 cd/m2). The following parameters were analyzed for the 4.00 cd.s/m2 stimulation: b-wave amplitude and implicit time, photopic negative response (PhNR) amplitude and implicit time, and PhNR/b-wave amplitude ratio. Weekly diurnal intraocular pressures and ophthalmic & physical exams were performed. Data were compared between groups using a repeated measures design, fitting a linear mixed model while controlling for baseline measurements.

Results : Treatment groups were found to be balanced and adequately randomized on baseline characteristics, such as age, pupil size, intraocular pressure, and sex. There was no detectable significant treatment effect of NAM for any of the tested ERG parameters. After 7 weeks of supplementation, the following values were obtained (mean ± SD) for treatment Group 1 vs control Group 2: b-wave amplitude, 15.8 ± 5.4 vs 29.7 ± 6.7 µV; b-wave implicit time, 31.9 ± 1.0 vs 30.5 ± 0.7 ms; PhNR amplitude, 18.7 ± 7.0 vs 22.8 ± 7.7µV; PhNR implicit time, 43.6 ± 3.7 vs 44.1 ± 2.1 ms; and PhNR/b-wave amplitude ratio, 1.3 ± 0.6 vs 0.8 ± 0.2.

Conclusions : Short-term NAM supplementation at selected standard doses did not result in a noticeable improvement of inner retinal function in the well-established, clinically relevant large animal OAG model. Larger sample sizes and longer treatment duration may be needed to enhance function in glaucomatous eyes and detect a neuroprotective effect.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×