Abstract
Purpose :
To compare cell counts on two anterior segment optical coherence tomography (AS-OCT) imaging devices using different scan protocols and parameters.
Methods :
Patients were prospectively imaged on two AS-OCT devices (Heidelberg Spectralis and Optovue Avanti) from 2020 to 2022 to compare cell counts in the anterior chamber. 10 eyes were included from 5 patients with active anterior segment inflammation. The Optovue images were captured using the standard 3D cornea settings with the image centered on the anterior chamber. Optovue AS-OCT images were analyzed using a proprietary software algorithm that has been described before to calculate cell density in the anterior chamber. Heidelberg images were captured using four settings for ART (1, 2, 4, and 9) to determine the effect of image averaging on cell counts/image quality. Heidelberg AS-OCT images were manually corrected slice by slice by an expert reader and the software calculated cell density in the anterior chamber. Pearson correlation coefficients of the Optovue and Heidelberg images with the various ART settings were calculated and compared. Correlation of clinical exam to AS-OCT cell density was also performed.
Results :
The average cell density on Optovue (33.761 cells/mm3) was much greater than the cell density on Heidelberg for all ART settings, ART 1 (3.192 cells/mm3), ART 2 (2.723 cells/mm3), ART 4 (5.87 cells/mm3), and ART 9 (8.872 cells/mm3). The Optovue displayed the best correlation to the fluid density at ART 2 (0.708) compared to the other ART settings, ART 1 (0.586), ART 4 (0.570), and ART 9 (0.584). The Pearson correlation coefficient comparing the uveitis clinical specialist grade and Optovue was 0.767 which compares to prior studies. Uveitis clinical specialist grade displayed the best correlation at ART 2 (0.713) compared to all other settings, ART 1 (0.559), ART 4 (0.603), and ART 9 (0.584).
Conclusions :
Heidelberg AS-OCT imaging has a moderate correlation to Optovue AS-OCT images when quantifying the degree of activity in anterior uveitis with the best correlation at ART 2. On average the cell density on Heidelberg was significantly lower than the cell density seen on Optovue AS-OCT images. The Heidelberg ART 2 displayed similar correlations with the Optovue with the uveitis clinical specialist grade. Thus, the use of the Heidelberg ART 2 setting may be used as an alternative to Optovue in objectively quantifying anterior segment inflammation.
This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.