June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Ultrafast ultrasound imaging of the trigeminal ganglion and brain in mice
Author Affiliations & Notes
  • Laurence Bourgeais Rambur
    Institut de la vision, Paris, Île-de-France, France
    Universite Paris Cite, Paris, Île-de-France, France
  • Youenn Travert
    Physics for Medicine, France
  • Felipe Cybis-Pereira
    Physics for Medicine, France
  • Jian Huang
    Institut de la vision, Paris, Île-de-France, France
  • Christophe Baudouin
    Institut de la vision, Paris, Île-de-France, France
    Hopital des 15-20, France
  • Stéphane melik-parsadaniantz
    Institut de la vision, Paris, Île-de-France, France
  • thomas deffieux
    Physics for Medicine, France
  • Sophie Pezet
    Physics for Medicine, France
  • Annabelle Reaux Le Goazigo
    Institut de la vision, Paris, Île-de-France, France
  • Footnotes
    Commercial Relationships   Laurence Bourgeais Rambur None; Youenn Travert None; Felipe Cybis-Pereira None; Jian Huang None; Christophe Baudouin None; Stéphane melik-parsadaniantz None; thomas deffieux None; Sophie Pezet None; Annabelle Reaux Le Goazigo None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 3361. doi:
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      Laurence Bourgeais Rambur, Youenn Travert, Felipe Cybis-Pereira, Jian Huang, Christophe Baudouin, Stéphane melik-parsadaniantz, thomas deffieux, Sophie Pezet, Annabelle Reaux Le Goazigo; Ultrafast ultrasound imaging of the trigeminal ganglion and brain in mice. Invest. Ophthalmol. Vis. Sci. 2023;64(8):3361.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The functional imaging within the trigeminal ganglion (TG) is highly challenging due to its small size and its deep localization. Because its high spatial and temporal sensitivity, functional ultrasound imaging (fUS) is a method of choice for non-invasive visualization of the TG and brain. We previously demonstrated that fUS imaging of TG and rat brain is feasible with craniotomy. Aim: This study aimed at finding the optimal anesthetic for the imaging session, imaging the TG vasculature through intact skull, studying the functional connectivity between the TG and these brain regions.

Methods : fUS imaging of TG was done in anesthetized adult male mice. Several anesthetics were tested during imaging session. A linear 2D fUS probe was placed on the intact skull of mice. Sagittal and coronal TG imaging sessions were performed. The software IcoStudio (Iconeus) was used for signal analysis.

Results : Anesthesia using 0.5% Isoflurane and intramuscular 0.1 mg/kg/h injection of Medetomidine was used for the imaging sessions in mice. We found that fUS imaging through the whole brain depth allowed a clear detection of bilateral TGs. Moreover, image analysis confirmed a high vascularization of the mice TG. Finally, we applied the vascular ‘brain positioning system’ for cross-referencing angiographic images to the Allen Mouse Brain Atlas to study functional connectivity between brain regions.

Conclusions : We provided the first proof of concept of imaging the TG using fUS imaging in mice with intact skull. This innovative imaging technology will allow the quantification of hemodynamic responses in the TG and whole brain induced by corneal stimulations.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

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