June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Connecting cilium membrane proteins facilitate Rhodopsin outer segment transport
Author Affiliations & Notes
  • Chunqiao Liu
    Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology, Guangzhou, Guangdong, China
  • Dianlei Guo
    Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology, Guangzhou, Guangdong, China
  • Lijing Xie
    Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology, Guangzhou, Guangdong, China
  • Mingjuan Wu
    Sun Yat-Sen University Zhongshan Ophthalmic Center State Key Laboratory of Ophthalmology, Guangzhou, Guangdong, China
  • Footnotes
    Commercial Relationships   Chunqiao Liu None; Dianlei Guo None; Lijing Xie None; Mingjuan Wu None
  • Footnotes
    Support   National Natural Science Foundation of China (NSFC; 31571077; Beijing, China), the Guangzhou City Sciences and Technologies Innovation Project (201707020009; Guangzhou, Guangdong Province, China)
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 3224. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Chunqiao Liu, Dianlei Guo, Lijing Xie, Mingjuan Wu; Connecting cilium membrane proteins facilitate Rhodopsin outer segment transport. Invest. Ophthalmol. Vis. Sci. 2023;64(8):3224.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : The connecting cilium (CC) of the photoreceptor provides the only physical route for the trafficking of the outer segment (OS) proteins. Despite the narrowness (~0.3um) of the CC bridge, it resides a wealth of proteins, disruption of which often leads to retinal degeneration. Among these proteins, the role of CC membrane proteins in OS protein trafficking has rarely been addressed. Our investigation aims to understand the role of CC membrane proteins in Rhodopsin
trafficking.

Methods : Immunohistochemistry was used to determine the CC protein and Rhodopsin localization. Genetically-engineered mutant mice were used to study CC membrane protein function. Protein-protein interactions were detected by pull-down experiment. The ciliary membrane protein complex was analyzed with TAP/MS. Expansion microscopy was conducted to visualize connecting cilium.

Results : Rhodopsin interacts with several ciliary membrane proteins including Tmem138 and Tmem231. Rhodopsin was detected partially colocalized with Tmem138 and Tmem231 at the CC area during development. Mislocalization of Rhodopsin was observed in Tmem138 mutants before the initiation of the outer segment. Protein interaction and TAP/MS assay identify several types of modified tubulins interacting with Tmem138, which might be involved in Rhodopsin transport.

Conclusions : Connecting ciliary membrane proteins could facilitate Rhodopsin OS transport by transient interaction with it and coordinating ciliary cytoplasm proteins and microtubules.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×