June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
The role of TGFbR1 signal in corneal development and homeostasis
Author Affiliations & Notes
  • Shingo Yasuda
    Wakayama Medical University, Japan
    College of Medicine University of Cincinnati, Ohio, United States
  • Yong Yuan
    College of Medicine University of Cincinnati, Ohio, United States
  • Jianhua Zhang
    College of Medicine University of Cincinnati, Ohio, United States
  • Yen-Chiao Wang
    Indiana University School of Medicine, Indianapolis, Indiana, United States
  • Sachin Anil
    Indiana University School of Medicine, Indianapolis, Indiana, United States
  • Winston Kao
    College of Medicine University of Cincinnati, Ohio, United States
  • Shizuya Saika
    Wakayama Medical University, Japan
  • chia-yang liu
    College of Medicine University of Cincinnati, Ohio, United States
    Indiana University School of Medicine, Indianapolis, Indiana, United States
  • Footnotes
    Commercial Relationships   Shingo Yasuda None; Yong Yuan None; Jianhua Zhang None; Yen-Chiao Wang None; Sachin Anil None; Winston Kao None; Shizuya Saika None; chia-yang liu None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 3163. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Shingo Yasuda, Yong Yuan, Jianhua Zhang, Yen-Chiao Wang, Sachin Anil, Winston Kao, Shizuya Saika, chia-yang liu; The role of TGFbR1 signal in corneal development and homeostasis. Invest. Ophthalmol. Vis. Sci. 2023;64(8):3163.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : TGFb is critical for cell proliferation, differentiation, and development. We made Alk5 (type1 TGFb receptor) conditional knockout in the cornea stroma (cst)to study roles of Alk5 during cornea development in the mice. This Alk5Δ/Δcst mice which induced doxycycline (dox) from E0 showed thin cornea and penetrated at P18-21 (Yasuda et al. ARVO 2019). In this study, we induced dox from postnate to estimate the role of corneal homeostasis.

Methods : 1) We stained E16.5, P0, P21, P56 WT mice cornea by TGFbR1 immunostaining to check where TGFbR1 appear. 2) We induced Alk5Δ/Δcst mice by dox from P33 to P90 and stained by HE and TGFbR1 immunostaining.

Results : 1) At E16.5 WT mice cornea, TGFbR1 appeared in epithelium, stroma and endothelium cells. On the other hands, TGFbR1 weakly detected in stromal keratocytes while both epithelial and endothelial cells yield strong fluorescence at P14 and P56. 2) Alk5Δ/Δcst mice induced by dox from P33 to P90 had normal thickness of cornea.

Conclusions : Alk5 signaling is important for development of mice cornea stroma, but may not be for homeostasis of cornea stroma.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×