June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
WITHDRAWN_Blimp1 maintains corneal homeostasis via regulating the differentiation of corneal epithelial cells
Author Affiliations & Notes
  • Xiaodong Liu
    Eye Institute of Xiamen University, Xiamen University School of Medicine, Xiamen, Fujian, China
    Xiamen University Xiamen Eye Center, Xiamen, Fujian, China
  • shangkun ou
    Eye Institute of Xiamen University, Xiamen University School of Medicine, Xiamen, Fujian, China
    Xiamen University Xiamen Eye Center, Xiamen, Fujian, China
  • Shengpeng Zhang
    Eye Institute of Xiamen University, Xiamen University School of Medicine, Xiamen, Fujian, China
    Xiamen University Xiamen Eye Center, Xiamen, Fujian, China
  • Wei Li
    Eye Institute of Xiamen University, Xiamen University School of Medicine, Xiamen, Fujian, China
    Xiamen University Xiamen Eye Center, Xiamen, Fujian, China
  • Footnotes
    Commercial Relationships   Xiaodong Liu None; shangkun ou None; Shengpeng Zhang None; Wei Li None
  • Footnotes
    Support  National Natural Science Foundation of China (NSFC; No. 81770894[to W.L.], No. 81970773 [to W.L.], No.82101084 [to S.O.], No. 81870625 [to R.Z.]), National Key R&D Program of China (2018YFA0107301 [to W.L.]), the China Postdoctoral Science Foundation (2021M69898 [to S.O.])
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 3147. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Xiaodong Liu, shangkun ou, Shengpeng Zhang, Wei Li; WITHDRAWN_Blimp1 maintains corneal homeostasis via regulating the differentiation of corneal epithelial cells. Invest. Ophthalmol. Vis. Sci. 2023;64(8):3147.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To explore the expression of B lymphocyte maturation-induced protein-1 (Blimp1) in corneal epithelium and evaluate its effects on the corneal epithelial homeostasis.

Methods : Fluorescence in situ hybridization (FISH) technique was used to detect the Blimp1 expression in rat cornea. The RNAi silencing of Blimp1 was achieved by adeno-associated virus (AAV) delivery of Blimp1-shRNA in primary human limbal epithelial cells. K12, K15, P63 and ABCG2 expression were evaluated by quantitative real-time PCR (qRT-PCR). Ocular surface changes of Blimp1 gene conditional knock-out (CKO) mice (K12-Cre×Blimp1-floxp/floxp) were observed under a slit-lamp microscope. RNA-seq was conducted to compare the gene expression and associated signal pathways between Blimp1 CKO and wild type mice cornea.

Results : Blimp1 was highly expressed in central corneal epithelium compared to limbus, mainly in terminal differentiated cells. K12 was down-regulated while K15, P63 and ABCG2 expression was up-regulated after Blimp1 knockdown. The corneal thickness was increased and ZO-1 was decreased in Blimp1 CKO mice. Fluorescein staining showed impaired corneal barrier function of Blimp1 CKO mice at 3 months. RNA-seq results suggested that NOD-like receptor signaling pathway, tight junction and cellular senescence related genes were involved in corneal epithelial changes after Blimp1 deletion.

Conclusions : Blimp1 plays a crucial role in maintaining the corneal epithelial homeostasis via regulating the differentiation of corneal epithelial cells.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×