Abstract
Presentation Description :
New advances in high resolution ophthalmoscopy have enabled the exciting prospect of directly imaging immune cell function in the living eye. Using adaptive optics ophthalmoscopy with phase-contrast, we have shown the detailed behaviors of microscopic immune cells and changes in blood flow in response to acute retinal inflammation in mice. These changes at the single-cell level are tracked from milliseconds-to-months as animals progress through the natural history of the inflammatory challenge. Using several mouse models of inflammation, we reveal the once hidden behaviors of immune cell activity without requiring fluorescent dyes or ex vivo preparations to study the spatially and temporally complex evolution of the retinal response to injury. This presentation will focus on the relationship between single-cell blood flow and the process by which circulating immune cells stick, roll and eventually extravasate through the vascular wall to arrive at the location of inflammation.
This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.