June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
In vivo quasi-elastic light scattering eye scanner detects molecular aging in humans and mice
Author Affiliations & Notes
  • Lee E Goldstein
    Department of Radiology, Boston University, Boston, Massachusetts, United States
    Boston University Alzheimer's Disease Center, Boston, Massachusetts, United States
  • Douglas Parsons
    Department of Radiology, Boston University, Boston, Massachusetts, United States
  • Srikant Sarangi
    Department of Biological Engineering, Boston University College of Engineering, Boston, Massachusetts, United States
  • Olga Minaeva
    Department of Radiology, Boston University, Boston, Massachusetts, United States
  • Danielle Ledoux
    Department of Ophthalmology, Boston Children's Hospital, Boston, Massachusetts, United States
    Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States
  • Juliet A Moncaster
    Department of Radiology, Boston University, Boston, Massachusetts, United States
  • John Clark
    Department of Biological Structure, University of Washington, Seattle, Washington, United States
  • David G Hunter
    Department of Ophthalmology, Boston Children's Hospital, Boston, Massachusetts, United States
    Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Lee Goldstein None; Douglas Parsons None; Srikant Sarangi None; Olga Minaeva None; Danielle Ledoux None; Juliet Moncaster None; John Clark None; David Hunter None
  • Footnotes
    Support  NIH/NIA Grant RF1AG072589
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 4394. doi:
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    • Get Citation

      Lee E Goldstein, Douglas Parsons, Srikant Sarangi, Olga Minaeva, Danielle Ledoux, Juliet A Moncaster, John Clark, David G Hunter; In vivo quasi-elastic light scattering eye scanner detects molecular aging in humans and mice. Invest. Ophthalmol. Vis. Sci. 2023;64(8):4394.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The absence of clinical tools to evaluate individual variation in the pace of aging represents a major impediment to understanding the biology of aging, assessing risk for aging-related diseases, and maximizing health throughout life (“healthspan”). The lens is an ideal tissue for quantitative assessment of molecular aging in vivo. We sought to measure aging-dependent signals in vivo in lenses of healthy human subjects and wild-type C57BL/6 mice. We hypothesize that cumulative alterations in long-lived lens proteins represent in vivo biomarkers of molecular aging in vivo.

Methods : We used in vivo quasi-elastic light scattering (QLS) to measure aging-dependent changes in clear lenses of 34 healthy humans without history of eye disease (18 males, 16 females; ages 5–61 years) and longitudinally in unanesthetized C57BL/6 mice with clear lenses (ages 4–16 months). We also examined time- and oxidation-dependent effects on QLS signals in a closed system of water-soluble human lens protein extract (hLPE) during long-term (~1 year) incubation in vitro.

Results : Our results show that aging-related QLS signals in the lens can be acquired noninvasively in human subjects and unanesthetized mice. Age-dependent QLS signal changes detected in vivo in humans and mice recapitulated time-dependent changes in hydrodynamic radius, protein polydispersity, and supramolecular order of human lens proteins during long-term incubation (~1 year) and in response to sustained oxidation (~2.5 months) in vitro.

Conclusions : Our findings demonstrate that QLS analysis of lens proteins provides a practical quantitative technique for noninvasive assessment of molecular aging in vivo.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

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