June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
The Effect of Total-body Irradiation on the Ocular Biometry and Intraocular Pressures in a Rabbit Model
Author Affiliations & Notes
  • He Eun Forbes
    Ophthalmology, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Aashka Damani
    Ophthalmology, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Sarah Kim
    Ophthalmology, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Shih-En Chen
    Ophthalmology, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Bhakti Panchal
    Ophthalmology, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Eduardo Faria
    Department of Radiation Oncology Division of translational radiation sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Sarah Triesler
    Department of Radiation Oncology Division of translational radiation sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Lindzy Calfee
    Department of Radiation Oncology Division of translational radiation sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Anthony Nuon
    Department of Radiation Oncology Division of translational radiation sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Osamah Saeedi
    Ophthalmology, University of Maryland School of Medicine, Baltimore, Maryland, United States
  • Footnotes
    Commercial Relationships   He Forbes None; Aashka Damani None; Sarah Kim None; Shih-En Chen None; Bhakti Panchal None; Eduardo Faria None; Sarah Triesler None; Lindzy Calfee None; Anthony Nuon None; Osamah Saeedi Vasoptic Inc., Code F (Financial Support), Heidelberg Engineering, Topcon, Code R (Recipient)
  • Footnotes
    Support  This project has been funded in whole or in part with Federal funds from the Administration for Strategic Preparedness and Response, Biomedical Advanced Research and Development Authority, under Contract No. HHSO100201500009I.
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 4986. doi:
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      He Eun Forbes, Aashka Damani, Sarah Kim, Shih-En Chen, Bhakti Panchal, Eduardo Faria, Sarah Triesler, Lindzy Calfee, Anthony Nuon, Osamah Saeedi; The Effect of Total-body Irradiation on the Ocular Biometry and Intraocular Pressures in a Rabbit Model. Invest. Ophthalmol. Vis. Sci. 2023;64(8):4986.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Total Body Irradiation is known to cause ocular complications such as cataract formation and microvascular retinal injury, but the acute effects of total body irradiation on ocular biometric metrics or intraocular pressure are not known. The purpose of this study is to investigate the acute effects of radiation on axial length, keratometry, and intraocular pressure (IOP) in an experimental model of total body irradiation of New Zealand White rabbits.

Methods : Axial length (AL), corneal curvature (K) and IOP were measured at baseline and serially after total body irradiation at 7.25 Gy in eleven male New Zealand White rabbits. The rabbits were irradiated at day 0 and subsequent measurements were taken at days 1, 5, 10, 15, 20, 25, and 30 following radiation or until euthanasia criteria were met. The AL and K-values were consecutively measured with a portable contact ultrasound tonometer and keratometer (PalmScan, Micro Medical Devices, Calabasas California) until the standard deviation was at an acceptable range of <=0.2. IOP was measured with a Tonopen AVIA (Reichert, Depew, NY) and if the statistical confidence indicator of the TPA was below 95% the measurement was repeated.

Results : The axial length was 15.7+/-0.3 mm at baseline and decreased significantly at day 1 (15.3+/-0.2 mm, p < 0.01) and day 5 (15.4 +/- 016 mm, p < 0.01) post-irradiation. It was decreased at day 10 (15.4 +/- 0.22 mm, p = 0.07), although it did not meet statistical significance. Keratometry was 45.6 +/- 1.5 diopters at baseline and was similar at day 1 (45.6 +/-1.8 D, p = 0.89), but significantly flatter at days 5 (44.7 +/- 1.7, p = 0.03) and 10 (44.3 +/- 1.5, p < 0.01). The baseline IOP was 8.8 +/- 1.1 mmHg and there was no significant difference in IOP at day 5 (7.8 +/- 1.5 mmHg, p = 0.4) or day 10 (8.4 +/- 1.7 mmHg, p = 1).

Conclusions : In a rabbit model, whole body irradiation resulted in shorter axial length and flatter corneal curvature at days 5 and 10. This is the first study to our knowledge to show changes in biometry with whole body irradiation. We hypothesize that this may be related to refractive changes associated with spaceflight.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

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