June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
MUSTARD GAS EXPOSURE IMPAIRS VISION VIA ALTERATIONS IN CORNEAL STROMAL ARCHITECTURE AND FIBROTIC GENES IN VIVO
Author Affiliations & Notes
  • Laila Suleiman
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri System, Columbia, Missouri, United States
    Harry S Truman Memorial Veterans' Hospital, Columbia, Missouri, United States
  • Nishant R Sinha
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri System, Columbia, Missouri, United States
    Harry S Truman Memorial Veterans' Hospital, Columbia, Missouri, United States
  • Ratnakar Tripathi
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri System, Columbia, Missouri, United States
  • Alexandria Hofmann
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri System, Columbia, Missouri, United States
  • Praveen Balne
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri System, Columbia, Missouri, United States
    Harry S Truman Memorial Veterans' Hospital, Columbia, Missouri, United States
  • Andrew C Holmes
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri System, Columbia, Missouri, United States
  • Riley Laub
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri System, Columbia, Missouri, United States
  • Shyam Chaurasia
    Departments of VMTH and Biomedical Sciences, College of Veterinary Medicine, University of Missouri System, Columbia, Missouri, United States
    Harry S Truman Memorial Veterans' Hospital, Columbia, Missouri, United States
  • Rajiv R Mohan
    School of Medicine, MU Health Care Mason Eye Clinic-University Hospital, Columbia, Missouri, United States
    Harry S Truman Memorial Veterans' Hospital, Columbia, Missouri, United States
  • Footnotes
    Commercial Relationships   Laila Suleiman None; Nishant Sinha None; Ratnakar Tripathi None; Alexandria Hofmann None; Praveen Balne None; Andrew Holmes None; Riley Laub None; Shyam Chaurasia None; Rajiv Mohan None
  • Footnotes
    Support  UO1 EY031650, NEI, NIH, RO1 EY030774, NEI, NIH, BX000357 VA Merit Award, IK6BX005646 VA Merit Award
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 4606. doi:
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    • Get Citation

      Laila Suleiman, Nishant R Sinha, Ratnakar Tripathi, Alexandria Hofmann, Praveen Balne, Andrew C Holmes, Riley Laub, Shyam Chaurasia, Rajiv R Mohan; MUSTARD GAS EXPOSURE IMPAIRS VISION VIA ALTERATIONS IN CORNEAL STROMAL ARCHITECTURE AND FIBROTIC GENES IN VIVO. Invest. Ophthalmol. Vis. Sci. 2023;64(8):4606.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Sulfur mustard gas (SM), a chemical weapon, has been used in many wars and most recently in Syrian conflict. SM exposure to disrupts the corneal stroma that constitutes 90% of the cornea. The characteristic collagen fibrils arrangement is necessary for corneal transparency and clear vision. This study evaluated changes in stromal collagen fibrils arrangement in post SM in rabbit eyes with transmission electron microscopy (TEM) and its impact on vision in live rabbits with 2D and 3D clinical eye imaging.

Methods : Fifty-four New Zealand White Rabbits divided into three groups (Naïve, Vehicle, SM-Vapor) were exposed to SM. At day-3, day-7, and day-14, eyes were examined/imaged in vivo with stereomicroscopy, slit-lamp, optical coherence tomography and confocal microscopes. Post euthanasia, H&E and Picrosirius Red staining to determine stromal integrity and collagen levels and TEM analysis were conducted at 50,000x to quantify collagen size and distance.

Results : Clinical eye exams of SM exposed rabbit eyes demonstrated significantly increased corneal haze (p<0.0001), thickness (p<0.0001), and debris (p<0.1) in stroma and significantly reduced stromal cell density (p<0.0001) compared to naïve corneas at all tested timepoints. TEM showed significantly altered arrangement of collagen fibrils in stroma of SM-exposed eyes at tested times (p<0.0001 or p<0.001). H&E and Picrosirius Red staining supported clinical findings with notable damage to the corneal epithelial stromal barrier at tested times.

Conclusions : Acute vision loss from mustard gas occurs via alterations in characteristic collagen fibrils arrangement in corneal stroma and changes in key fibrotic markers, TGFb and collagens.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

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