June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Inflammatory cytokines levels after intraocular silicone oil endotamponade
Author Affiliations & Notes
  • Jose-Carlos Pastor
    IOBA, IOBA-University of Valladolid, Valladolid, Valladolid, Spain
  • Francisco-Javier Valentin-Bravo
    IOBA, IOBA-University of Valladolid, Valladolid, Valladolid, Spain
    Ophthalmology, Hospital Clinico Universitario de Valladolid, Valladolid, Castilla y León, Spain
  • Eduardo Valentin-Bravo
    IOBA, IOBA-University of Valladolid, Valladolid, Valladolid, Spain
    Ophthalmology, Hospital Clinico Universitario de Valladolid, Valladolid, Castilla y León, Spain
  • Eva Sobas-Abad
    IOBA, IOBA-University of Valladolid, Valladolid, Valladolid, Spain
    Nursing School, Universidad de Valladolid, Valladolid, Castilla y León, Spain
  • Carmen Garcia-Vazquez
    IOBA, IOBA-University of Valladolid, Valladolid, Valladolid, Spain
  • Nadia Galindo-Cabello
    IOBA, IOBA-University of Valladolid, Valladolid, Valladolid, Spain
  • Cristina Andres-Iglesias
    IOBA, IOBA-University of Valladolid, Valladolid, Valladolid, Spain
  • Ricardo Usategui-Martin
    IOBA, IOBA-University of Valladolid, Valladolid, Valladolid, Spain
    Cell Biology, Universidad de Valladolid, Valladolid, Castilla y León, Spain
  • Salvador Pastor-Idoate
    IOBA, IOBA-University of Valladolid, Valladolid, Valladolid, Spain
    Ophthalmology, Hospital Clinico Universitario de Valladolid, Valladolid, Castilla y León, Spain
  • Footnotes
    Commercial Relationships   Jose-Carlos Pastor None; Francisco-Javier Valentin-Bravo None; Eduardo Valentin-Bravo None; Eva Sobas-Abad None; Carmen Garcia-Vazquez None; Nadia Galindo-Cabello None; Cristina Andres-Iglesias None; Ricardo Usategui-Martin None; Salvador Pastor-Idoate None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 5253. doi:
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      Jose-Carlos Pastor, Francisco-Javier Valentin-Bravo, Eduardo Valentin-Bravo, Eva Sobas-Abad, Carmen Garcia-Vazquez, Nadia Galindo-Cabello, Cristina Andres-Iglesias, Ricardo Usategui-Martin, Salvador Pastor-Idoate; Inflammatory cytokines levels after intraocular silicone oil endotamponade. Invest. Ophthalmol. Vis. Sci. 2023;64(8):5253.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Intraocular silicone oil (SiO) is related to multiple side effects by its lipophilic nature and ability to emulsify but it is also beyond doubt that SiO produces both acute and chronic intraocular inflammatory reactions.
This study tries to objective determine the expression of several inflammatory cytokines in the silicone oil (SiO) supernatant extracted from patients with retinal detachments (RD), to elucidate whether they are related to either the time of intraocular permanence or viscosity of SiO.

Methods : Research was approved by Valladolid Ethics Committee (Ref PI-GR 20-1987). Prospective study in 25 consecutive patients who had RD and underwent pars plana vitrectomy using SiO was done. An analysis of SO supernatant was performed by multiplex bead immunoassay Luminex IS-100 (Luminex Corporation, Austin, TX). Inflammation-related cytokines and proteins included: IL1α, IL4, IL6, IL8, IL10, IL12, IL17, IP10/CXCL10, INFγ, TNFα,TGFβ1, TGFβ2 and TGFβ3. Fifteen patients with 1000 centistokes SiO (SIOBAL 1000, AJL, Vitoria Spain) and 10 patients with 5000 cs SiO (SIOBAL 5000) were analyzed. Results were compared using the Mann–Whitney U test. All analyses were performed with SPSS version 22.0 statistical package (SPSS, Chicago, IL, USA).

Results : Mean time of SO endotamponade was 5.48 (±4.44) months. SiO was removed before 3 months in 9 patients and lasted more than 3 months in 16 . Only few cytokines were detectable: IL6, IL8, IL10, TGFβ1, TGFβ2 and TGFβ3. Values according to the endotamponade time (months) (mean and SD in pg/mL) were:IL-6 < 3 m 34,1 (30,6); >3m 28,1 (30,0) p 0,64; IL-8 <3m 20,4 (14,8); >3m 19,3(26,4) p 0,92; IL-10 <3m 68,1(20,1);>3m 58,1 (30,2) p 0,39; TGFβ1 <3m 21,9 (11,0); >3m 27,9 (25,8) p 0,52; TGFβ2 <3m 141,2 (52,7);>3m 129,7 (66,1) p 0,66; TGFβ3 <3m 12,03 (16,6); >3 m 16,3 (20,1) p 0,62. Concentrations of cytokines and SiO viscosity (1000 and 5000 cs) were IL-6 25,5 (29,2); 33.0 (31,1) p 0,62; IL-8 17,9 (18,5); 21,0 (25,4) p 0,75; IL-10 51,8 (27,9); 69,8 (23,8) p 0,11; TGFβ1 27,6 (25,9); 24,3 (18,4) p 0,72; TGFβ2 129,1 (49,9); 138,1 (68,7) p0,73; TGFβ3 13,3 (16,2);15,5 (20,5) p 0,8

Conclusions : Although the great variability and the small sample size do not allow conclusions to be drawn, there is a tendency that with shorter endotamponade time and with more viscous SiO there is a higher inflammatory response. These data must be confirmed

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

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