June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Visual Electrophysiologic Correlates of Intra-uterine Exposure to ZIKA Virus in a Non-human Primate Model
Author Affiliations & Notes
  • James N Ver Hoeve
    Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States
  • Charlene B Y Kim
    Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States
  • Nick Krabbe
    Pediatrics, University of Wisconsin-Madison, Madison, Wisconsin, United States
  • Elaina Razo
    Pediatrics, University of Wisconsin-Madison, Madison, Wisconsin, United States
  • T Michael Nork
    Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States
  • Carol Rasmussen
    Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States
  • Alexander Katz
    Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States
  • Karla Ausdernau
    Pediatrics, University of Wisconsin-Madison, Madison, Wisconsin, United States
  • Emma Mohr
    Pediatrics, University of Wisconsin-Madison, Madison, Wisconsin, United States
  • Footnotes
    Commercial Relationships   James Ver Hoeve None; Charlene Kim None; Nick Krabbe None; Elaina Razo None; T Michael Nork None; Carol Rasmussen OSOD, LLC, Code C (Consultant/Contractor); Alexander Katz None; Karla Ausdernau None; Emma Mohr None
  • Footnotes
    Support  NIH/NIAID (P01 AI132132);NIH/NIAID (R01 AI132519);NIH/NIAID (K08 AI139341);NIH/NIAID (R01 AI153130);NIH/NEI(P30 EY016665);Unrestricted Grant from Research to Prevent Blindness, Inc. to the UW-Madison Department of Ophthalmology and Visual Sciences.
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 5218. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      James N Ver Hoeve, Charlene B Y Kim, Nick Krabbe, Elaina Razo, T Michael Nork, Carol Rasmussen, Alexander Katz, Karla Ausdernau, Emma Mohr; Visual Electrophysiologic Correlates of Intra-uterine Exposure to ZIKA Virus in a Non-human Primate Model. Invest. Ophthalmol. Vis. Sci. 2023;64(8):5218.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To determine whether Zika virus (ZIKV) exposure (ZVE) during pregnancy in a non-human primate (NHP) model alters non-invasive measures of visual function.

Methods : Pregnant rhesus macaques were inoculated with ZIKV from Puerto Rico or Africa during the first trimester. Cesarean-delivered ZVE infants were compared with sham-injected controls. Infant assessments included: neurobehavioral evaluation, ophthalmic examinations (OE), optical coherence tomography (OCT) of the anterior and posterior retinal segments, electroretinography (ERG) and flash visual evoked potentials (FVEP). Additional details have been previously described (Koenig et al, PLOSOne, 2020). Functional assessment included serval classes of tests: photopic and scotopic ERG v. flash intensity series, monocular FVEP and 30 Hz flicker harmonics. Predictor variables included standard A- and B-wave measures, Naka-Rushton (N-R) fits, and FFT harmonics of flicker ERG and oscillatory potentials (OPs). Logistic regression was used to analyze the relationship between electrophysiologic measures and the probability of ZVE. Models using variables to predict exposure were evaluated within and across classes of electrophysiologic test at postnatal ages of 45, 90, and 360 days.

Results : No abnormalities in OE or OCT were noted in ZVE infants. At 45 days of age (N= 12 controls, 14 exposed), the likelihood of ZVE was higher in individuals with larger photopic A-wave amplitudes and longer implicit times. At 90 days of age (15 controls, 27 exposed), amplitude of the photopic B-wave, photopic negative response and FVEP predicted ZVE. At 360 days (18 controls, 26 exposed), models from several test classes significantly predicted ZVE including, amplitude of photopic monocular FVEP, 30 Hz ERG, and 30 Hz VEP; the goodness-of-fit of the N-R model for the photopic series FVEP; 30 Hz F3 ERG and F3 FVEP amplitude, and photopic and scotopic OPs. A model using the best predictors of ZVE from within each class significantly predicted ZVE (p < 0.01) with an AUC of 0.878, sensitivity of 0.846 and specificity of 0.722.

Conclusions : Although few abnormalities were noted in OCT imaging or OE, non-invasive functional measures were found that predicted ZVE status at each age. This suggests there are early and long-term sub-clinical changes in the retina and central visual pathways of ZVE infants.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×