June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Bioengineered Liquid Cornea: Promotes regeneration in Mechanical Injury Wound Model of Rabbits
Author Affiliations & Notes
  • Virender Singh Singh Sangwan
    Stem Cells and Cornea, Dr Shroff's Charity Eye Hospital Delhi, New Delhi, Delhi, India
  • Tuhin Bhowmick
    Pandorum technologies Pvt. Ltd, Bangalore, Please select, India
  • Mehak Vohra
    Pandorum technologies Pvt. Ltd, Bangalore, Please select, India
  • Anil Tiwari
    Stem Cells and Cornea, Dr Shroff's Charity Eye Hospital Delhi, New Delhi, Delhi, India
  • Abha Gour
    Stem Cells and Cornea, Dr Shroff's Charity Eye Hospital Delhi, New Delhi, Delhi, India
  • Jyoti Rajput
    Pandorum technologies Pvt. Ltd, Bangalore, Please select, India
  • Bharti Sangwan
    Pandorum technologies Pvt. Ltd, Bangalore, Please select, India
  • Kartik Goel
    Pandorum technologies Pvt. Ltd, Bangalore, Please select, India
  • Parinita Agrawal
    Pandorum technologies Pvt. Ltd, Bangalore, Please select, India
  • Utkarsh Bhutani
    Pandorum technologies Pvt. Ltd, Bangalore, Please select, India
  • Neha Waghmare
    Pandorum technologies Pvt. Ltd, Bangalore, Please select, India
  • Suvro K. Chowdhury
    Pandorum technologies Pvt. Ltd, Bangalore, Please select, India
  • Kamalnath Selvakumar
    Pandorum technologies Pvt. Ltd, Bangalore, Please select, India
  • Arun Chandru
    Pandorum technologies Pvt. Ltd, Bangalore, Please select, India
  • Ritu Raj
    Pandorum technologies Pvt. Ltd, Bangalore, Please select, India
  • Footnotes
    Commercial Relationships   Virender Singh Sangwan None; Tuhin Bhowmick Pandorum technologies Pvt Ltd, Code O (Owner); Mehak Vohra Pandorum technologies Pvt Ltd, Code E (Employment); Anil Tiwari None; Abha Gour None; Jyoti Rajput None; Bharti Sangwan None; Kartik Goel None; Parinita Agrawal None; Utkarsh Bhutani None; Neha Waghmare None; Suvro K. Chowdhury None; Kamalnath Selvakumar None; Arun Chandru Pandorum technologies Pvt Ltd, Code O (Owner); Ritu Raj Pandorum technologies Pvt Ltd, Code E (Employment)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 5147. doi:
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      Virender Singh Singh Sangwan, Tuhin Bhowmick, Mehak Vohra, Anil Tiwari, Abha Gour, Jyoti Rajput, Bharti Sangwan, Kartik Goel, Parinita Agrawal, Utkarsh Bhutani, Neha Waghmare, Suvro K. Chowdhury, Kamalnath Selvakumar, Arun Chandru, Ritu Raj; Bioengineered Liquid Cornea: Promotes regeneration in Mechanical Injury Wound Model of Rabbits. Invest. Ophthalmol. Vis. Sci. 2023;64(8):5147.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Loss of corneal transparency and poor refractive function are the leading causes of blindness. Corneal transplant is gold standard for management of corneal conditions. The balance between corneal donors and recipients is skewed towards recipient leading to extensive wait time. To address this, artificial cornea is being employed, where an injured cornea will be removed and replaced with liquid cornea for treatment.

Methods : Dual biopolymer-based formulation combination was optimized based on screening with different photoinitiator based systems. The biopolymer was engineered to be implanted in a minimally invasive manner. New Zealand Rabbits were used to perform pre-clinical efficacy study. Corneal Mechanical wound injury of 5mm diameter and 200um depth created using a graded trephine. Wounded animals were divided, first group received no treated (controls), second group, treated with tissue adhesive and third group, treated with bioengineered liquid cornea (biopolymer exosomes). Animals were imaged using the ophthalmological parameters; i) OCT, ii) Slit lamp and iii) Pentacam and iv) IVCM, over a period of 3 months. After 3 months, rabbits were sacrificed, and eyes were enucleated and further processed for histopatholog

Results : The hydrogels demonstrated 92% transparency and appropriate mechanical stiffness (250-350 kPa) comparable with the properties of native cornea. Possess high adhesion properties (30-40 kPa) rendering it suture-free capabilities, controlled swelling and degradation ensuring structural support till corneal regeneration. In rabbits, slit lamp reveled re-epithelization of the wound treated with bioengineered liquid cornea, within 15 days. Central Corneal thickness of control rabbits ranged around 350-360 um while bioengineered liquid cornea treated rabbits showed central corneal thickness (380-390um) almost like the control. In addition, the transparency of the regenerated cornea mimicked the native cornea as measured by opacity score.

Conclusions : The properties of a biopolymeric hydrogel substitute can be tuned for any specific application following the proposed critical factors of processing. The bioengineered liquid cornea is a regenerative treatment, and this biopolymer acts as a sacrificial matrix for accelerating the growth of host tissue to cover the wound site.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

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