Abstract
Purpose :
To evaluate linear corneal density measurements of normal corneas with spectral domain optical coherence tomography (OCT), using the Image J program.
Methods :
In a cross-sectional descriptive and observational study, spectral corneal OCT (Heidelberg Engineering) images obtained with the anterior OCT segment lens in healthy corneas were analyzed to determine an average density from each layer. We included subjects without corneal pathologies or surgeries, categorized according to age: 30-50 years, 51 to 70 years, and ≥70 years. Image analysis of densities was performed using a linear measurement with ImageJ, an open platform of the NIH.
Results :
Spectral corneal OCT images of 140 eyes (170 patients), were analyzed from 90 males and 80 females. We found that the average grey value density for each layer were: tear layer 231±22, epithelium interface 90±17 , basal membrane and Bowman 172±12, anterior stroma 156±14, posterior stroma 121±9 and Descemet membrane 154±9. Optical densities showed significant differences between each layer (p=0.002).
Conclusions :
LM-SD-OCT with Image J was able to provide corneal layer densities as a fast and practical method to provide complementary information of the subjective descriptions from spectral corneal OCT images.
This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.