June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Analysis of retinal blood flow dynamics with time-resolved structural optical coherence tomography
Author Affiliations & Notes
  • Philippe Valmaggia
    Department of Biomedical Engineering, Universitat Basel, Basel, Basel-Stadt, Switzerland
    Institute of Molecular and Clinical Ophthalmology Basel, Basel, Basel-Stadt, Switzerland
  • Philippe Claude Cattin
    Department of Biomedical Engineering, Universitat Basel, Basel, Basel-Stadt, Switzerland
  • Robin Sandkühler
    Department of Biomedical Engineering, Universitat Basel, Basel, Basel-Stadt, Switzerland
  • Tilman Otto
    Heidelberg Engineering GmbH, Heidelberg, Baden-Württemberg, Germany
  • Michel Teussink
    Heidelberg Engineering GmbH, Heidelberg, Baden-Württemberg, Germany
  • Silke Aumann
    Heidelberg Engineering GmbH, Heidelberg, Baden-Württemberg, Germany
  • Nadja Inglin
    Institute of Molecular and Clinical Ophthalmology Basel, Basel, Basel-Stadt, Switzerland
  • Hendrik P Scholl
    Institute of Molecular and Clinical Ophthalmology Basel, Basel, Basel-Stadt, Switzerland
  • Peter Maloca
    Institute of Molecular and Clinical Ophthalmology Basel, Basel, Basel-Stadt, Switzerland
  • Footnotes
    Commercial Relationships   Philippe Valmaggia Heidelberg Engineering, Code R (Recipient); Philippe Cattin None; Robin Sandkühler None; Tilman Otto Heidelberg Engineering, Code E (Employment); Michel Teussink Heidelberg Engineering, Code E (Employment); Silke Aumann Heidelberg Engineering, Code E (Employment); Nadja Inglin None; Hendrik Scholl None; Peter Maloca Heidelberg Engineering, Code R (Recipient)
  • Footnotes
    Support  SNSF Grant 323530_199395
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 3363. doi:
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      Philippe Valmaggia, Philippe Claude Cattin, Robin Sandkühler, Tilman Otto, Michel Teussink, Silke Aumann, Nadja Inglin, Hendrik P Scholl, Peter Maloca; Analysis of retinal blood flow dynamics with time-resolved structural optical coherence tomography. Invest. Ophthalmol. Vis. Sci. 2023;64(8):3363.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To present a method to analyse retinal blood flow dynamics using time-resolved structural optical coherence tomography (OCT).

Methods : We developed imaging protocols to acquire time-resolved OCT B-scans (1024 x 496 pixels, 10° field of view) at four different sensor integration times (7.24 μs, 11.2 μs, 22.4 μs, 44.8 μs). The acquired B-scans were aligned with rigid body transformations and matched to a time-axis with timestamps. The vessel centres were manually annotated for each B-scan and surrounding subvolumes were extracted. Fringe washout analysis was performed and used to calculate blood flow velocities in vessels within one optic disc diameter of the optic disc rim.

Results : Time-resolved structural OCT revealed pulsatile intensity changes in the analysed vessels. The fringe washout analysis showed higher signal differences in acquisitions with longer integration times. The fringe washout analysis enabled to calculate the axial components of the blood flow velocity. The pulsatile nature of the fringe washout indicates a correlation with the cardiac cycle and a representation of the pulse propagation in the vasculature of the optic nerve head.

Conclusions : In this study, we demonstrated the feasibility to calculate blood flow velocity profiles in vessels close to the optic nerve head using time-resolved structural OCT. The fringe washout analysis revealed pulsatile blood flow dynamics. Time-resolved structural OCT with its high spatial and temporal resolution holds promising information that could be further investigated as a new clinically applicable parameter for the assessment of blood flow velocities.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

 

(A) Time-resolved optical coherence tomography (OCT) B-scans of optic nerve head vessels. Imaging was performed at the same location. Two B-scans and eight extracted arterial subvolumes (7 x 7 pixels) are shown with timestamps (corresponding subvolumes framed in red). The intensity changes in the subvolume occur due to varying fringe washout of the arterial signal over time. (B) Analysis of retinal blood flow dynamics over 6 seconds in an artery with time-resolved OCT (A-scan integration time 22.4 μs, inter-B-scan interval 25 ms). Timestamp-matched mean intensity of the OCT subvolume (top row), minimal axial flow velocity (middle row), and calculated axial flow velocity values (bottom row).

(A) Time-resolved optical coherence tomography (OCT) B-scans of optic nerve head vessels. Imaging was performed at the same location. Two B-scans and eight extracted arterial subvolumes (7 x 7 pixels) are shown with timestamps (corresponding subvolumes framed in red). The intensity changes in the subvolume occur due to varying fringe washout of the arterial signal over time. (B) Analysis of retinal blood flow dynamics over 6 seconds in an artery with time-resolved OCT (A-scan integration time 22.4 μs, inter-B-scan interval 25 ms). Timestamp-matched mean intensity of the OCT subvolume (top row), minimal axial flow velocity (middle row), and calculated axial flow velocity values (bottom row).

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