June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Visible-light optical coherence microscopy
Author Affiliations & Notes
  • Shanjida Khan
    Biomedical Engineering, Oregon Health & Science University, Portland, Oregon, United States
    Oregon Health & Science University Casey Eye Institute, Portland, Oregon, United States
  • Kai Neuhaus
    Oregon Health & Science University Casey Eye Institute, Portland, Oregon, United States
  • Omkar C Thaware
    Biomedical Engineering, Oregon Health & Science University, Portland, Oregon, United States
    Oregon Health & Science University Casey Eye Institute, Portland, Oregon, United States
  • Mini Aga
    Oregon Health & Science University Casey Eye Institute, Portland, Oregon, United States
  • Alireza Karimi
    Department of Ophthalmology and Visual Sciences, The University of Alabama at Birmingham College of Arts and Sciences, Birmingham, Alabama, United States
  • Mary J Kelley
    Oregon Health & Science University Casey Eye Institute, Portland, Oregon, United States
  • Travis Redd
    Oregon Health & Science University Casey Eye Institute, Portland, Oregon, United States
  • Ted S Acott
    Oregon Health & Science University Casey Eye Institute, Portland, Oregon, United States
  • David Huang
    Biomedical Engineering, Oregon Health & Science University, Portland, Oregon, United States
    Oregon Health & Science University Casey Eye Institute, Portland, Oregon, United States
  • Yifan Jian
    Biomedical Engineering, Oregon Health & Science University, Portland, Oregon, United States
    Oregon Health & Science University Casey Eye Institute, Portland, Oregon, United States
  • Footnotes
    Commercial Relationships   Shanjida Khan None; Kai Neuhaus None; Omkar Thaware None; Mini Aga None; Alireza Karimi None; Mary Kelley None; Travis Redd None; Ted Acott None; David Huang Optovue Inc., Code F (Financial Support), Optovue Inc., Code I (Personal Financial Interest), Optovue Inc., Code P (Patent), Optovue Inc., Code R (Recipient); Yifan Jian Seymour Vision, Code O (Owner)
  • Footnotes
    Support  National Institutes of Health (P30 EY010572, R01 EY028755, R01HD107494, T32 EY023211); Oregon Health and Science University (unrestricted departmental grant); Research to Prevent Blindness (Career Advancement Award).
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 1966. doi:
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    • Get Citation

      Shanjida Khan, Kai Neuhaus, Omkar C Thaware, Mini Aga, Alireza Karimi, Mary J Kelley, Travis Redd, Ted S Acott, David Huang, Yifan Jian; Visible-light optical coherence microscopy. Invest. Ophthalmol. Vis. Sci. 2023;64(8):1966.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Corneal disease is the fifth leading cause of visual loss globally. Current clinical imaging applications such as in vivo confocal microscopy (IVCM) offer high lateral resolution to observe cellular structures. However, these clinical applications lack large field of view (FOV) and volumetric imaging capability, and are highly operator dependent. This observational study utilizes blue- and green-light optical coherence microscopy to image cellular structures on ex vivo samples as well as study pressure changes in anterior segment human donor eyes.

Methods : Two visible-light optical coherence microscopy (OCM) systems were used in this study. The blue- and green-light sources were at a center wavelength of 450 nm and 510 nm respectively. The OCM systems were based on a spectral domain optical coherence tomography engine, and achieved a FOV of up to 750 μm Χ 750 μm and a lateral resolution of 1.6 μm over a 1.2 mm imaging depth.

Results : Five enucleated adult white rabbit eyes were imaged with both OCM systems and compared with IVCM images. Epithelial cells, endothelial cells, and keratocytes were visualized (Fig. 1). Additionally, collagen lamellae with a clear, striated pattern were observed. For the pressure study, one end of a cannula was inserted into the Schlemm’s canal (SC) lumen of the anterior segment human eye wedge, and the other into a saline reservoir with a relative density similar to that of aqueous humor. The reservoir’s height was altered to create pressure changes in the SC lumen. En face and cross-sectional images of trabecular meshwork, which lies directly above the SC lumen, of the human eye wedge are depicted in Fig. 2.

Conclusions : We achieved well contrasted en face images with our visiblie-light OCM systems compared to IVCM,and were able to observe collagen lamellae density changes at different depths with the use of a variable focus liquid lens. With the pressure study model, we were able to observe SC lumen changes and use data to further analyze the biomechanics of the tissue.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

 

 

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