June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Metabolomic profiling of long-chain polyunsaturated fatty acid oxidation in patients with retinal vein occlusion
Author Affiliations & Notes
  • Yan Gong
    Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China
  • Anli Ren
    Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China
  • Jiangbo Ren
    Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China
  • Zhengrong Huang
    Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China
  • Xizhi Deng
    Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China
  • Ziyu Jiang
    Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China
  • Yanni Xue
    Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China
  • Zhongjie Fu
    Department of Ophthalmology, Boston Children's Hospital, Boston, Massachusetts, United States
  • Min Ke
    Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China
  • Lois Smith
    Department of Ophthalmology, Boston Children's Hospital, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Yan Gong None; Anli Ren None; Jiangbo Ren None; Zhengrong Huang None; Xizhi Deng None; Ziyu Jiang None; Yanni Xue None; Zhongjie Fu None; Min Ke None; Lois Smith None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 1763. doi:
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    • Get Citation

      Yan Gong, Anli Ren, Jiangbo Ren, Zhengrong Huang, Xizhi Deng, Ziyu Jiang, Yanni Xue, Zhongjie Fu, Min Ke, Lois Smith; Metabolomic profiling of long-chain polyunsaturated fatty acid oxidation in patients with retinal vein occlusion. Invest. Ophthalmol. Vis. Sci. 2023;64(8):1763.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The clinical significance of long-chain polyunsaturated fatty acids (LCPUFA) and their metabolites on retinal vein occlusion (RVO) remains unclear. This cross-sectional study aimed to explore metabolomic profiles of LCPUFA oxidation in RVO patients, and to identify potential confounders for diagnostic and prognostic prediction.

Methods : Venous blood samples were collected from 44 RVO patients and 36 controls. The plasma levels of ω-3 and ω-6 LCPUFA and their oxylipins were analyzed with ultra-performance liquid chromatography tandem mass spectrometry. Univariate analysis combined with principal component analysis and orthogonal projections to latent structure discriminant analysis was used to screen differential metabolites. Ex vivo aortic ring and choroidal explant sprouting assays were used to investigate the effects of 5-oxo-eicosatetraenoic acids (ETE) on angiogenesis. Tubule formation and wound healing assays were performed to verify the effects of 5-oxo-ETE on human retinal microvascular endothelial cell (HRMEC) functions.

Results : RVO patients had higher ω-6 and lower ω-3 LCPUFA levels in the plasma. Metabolomic analysis revealed 20 LCPUFA and their oxylipins dysregulated in the RVO patients, including increased arachidonic acid (ω-6, P < 0.05) and its lipoxygenase product 5-oxo-ETE with the most substantial change (P < 0.05), as well as decreased docosahexaenoic acid (ω-3, P < 0.05). Interestingly, the 5-oxo-ETE levels were lower in the ischemic vs. non-ischemic central RVO patients. Ex vivo tissue culture and in vitro HRMEC assays suggested that 5-oxo-ETE attenuated aortic ring and choroidal explant sprouting, and inhibited tubule formation and migration of HRMECs in a dose-dependent manner, possibly through suppressing the vascular endothelial growth factor-induced signaling pathways.

Conclusions : The plasma levels of ω-6 and ω-3 LCFPUA and their oxylipins were associated with RVO. The ω-6 LCPUFA-derived metabolite 5-oxo-ETE was a potential marker of RVO development and progression.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

 

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