June 2023
Volume 64, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2023
Comparative Localized Corneal Biomechanical Changes Following Corneal Cross-Linking Using Brillouin Microscopy
Author Affiliations & Notes
  • James Bradley Randleman
    Ophthalmology, Cleveland Clinic Cole Eye Institute, Cleveland, Ohio, United States
  • Lara Asroui
    Ophthalmology, Cleveland Clinic Cole Eye Institute, Cleveland, Ohio, United States
  • IMANE TARIB
    Ophthalmology, Cleveland Clinic Cole Eye Institute, Cleveland, Ohio, United States
  • Hongyuan Zhang
    Ophthalmology, Cleveland Clinic Cole Eye Institute, Cleveland, Ohio, United States
  • Giuliano Scarcelli
    Engineering, University System of Maryland, College Park, Maryland, United States
  • Footnotes
    Commercial Relationships   James Randleman None; Lara Asroui None; IMANE TARIB None; Hongyuan Zhang None; Giuliano Scarcelli None
  • Footnotes
    Support  R01 EY028666
Investigative Ophthalmology & Visual Science June 2023, Vol.64, 1683. doi:
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    • Get Citation

      James Bradley Randleman, Lara Asroui, IMANE TARIB, Hongyuan Zhang, Giuliano Scarcelli; Comparative Localized Corneal Biomechanical Changes Following Corneal Cross-Linking Using Brillouin Microscopy. Invest. Ophthalmol. Vis. Sci. 2023;64(8):1683.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To identify focal corneal biomechanical alterations in vivo after Standard Protocol CXL using motion-tracking Brillouin microscopy.

Methods : Prospective study comparing motion tracking Brillouin shift data from patients before and 3 months after undergoing CXL. Patients had both Scheimpflug imaging and motion tracking Brillouin microscopy imaging. Mean, Maximum (Max), and Minimum (Min) Brillouin shift, Spatial standard deviation (SSD), and Max-Min values were compared. The primary endpoint was the regional change in Brillouin longitudinal modulus (postoperatively (MHz) as measured within the central 6mm.

Results : Postoperatively, CXL induced stiffening across the central 6mm of the cornea, with mean Brillouin shift increasing by 20.42 ± 10.81 MHz. There was wide variation in induced stiffening across different patients, with individual Mean Brillouin shifts ranging from 11.94 ± 5.35 to 25.39 MHz ± 12.72 MHz.

Conclusions : Brillouin microscopy successfully measured focal corneal stiffness increases after CXL, thereby providing novel, highly specific localized corneal biomechanical data in vivo. The wide range of induced stiffening measured after standard protocol CXL mirrors the clinical variation seen after CXL procedures.

This abstract was presented at the 2023 ARVO Annual Meeting, held in New Orleans, LA, April 23-27, 2023.

 

Motion-tracking Brilloun microscopy difference map image showing the change in Brilloun shift as (in megaHertz) after Corneal Cross-linking. positive valuyes indice that cornea is stiffer after treatment

Motion-tracking Brilloun microscopy difference map image showing the change in Brilloun shift as (in megaHertz) after Corneal Cross-linking. positive valuyes indice that cornea is stiffer after treatment

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